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High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

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Title: High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima
Authors: Hisano, H. Browse this author
Kimoto, Y. Browse this author
Hayakawa, H. Browse this author
Takeichi, J. Browse this author
Domae, T. Browse this author
Hashimoto, R. Browse this author
Abe, J. Browse this author →KAKEN DB
Asano, S. Browse this author →KAKEN DB
Kanazawa, A. Browse this author →KAKEN DB
Shimamoto, Y. Browse this author
Keywords: Agrobacterium tumefaciens
Beta vulgaris
Regeneration
Shoot-base tissue
Transformation
Issue Date: Jul-2004
Publisher: Springer Berlin / Heidelberg
Journal Title: Plant Cell Reports
Volume: 22
Issue: 12
Start Page: 910
End Page: 918
Publisher DOI: 10.1007/s00299-004-0773-3
PMID: 15042407
Abstract: Communicated by H. Uchimiya Abstract We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and -glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.
Rights: The original publication is available at www.springerlink.com
Relation: http://www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/16870
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金澤 章

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