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Volume 54, Number 2-3 >

Development of ELISA to detect antibodies specific to Mycobacterium aviumsubsp. paratuberculosis with truncated34kDa proteins

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.54.2-3.99

Title: Development of ELISA to detect antibodies specific to Mycobacterium aviumsubsp. paratuberculosis with truncated34kDa proteins
Authors: Malamo, Mumeka Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Ozaki, Hiroichi Browse this author
Kida, Hiroshi Browse this author →KAKEN DB
Keywords: cattle
diagnosis
ELISA
mycobacterium
paratuberculosis
Issue Date: 30-Nov-2006
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 54
Issue: 2-3
Start Page: 99
End Page: 107
Abstract: To develop ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis ),the carboxyl termini of the 34 kDa proteins of M. paratuberculosis and Mycobacterium avium subsp. avium(M. avium)were expressed in Escherichia coli expression system. Antibodies specific to M. paratuberculosis were detected with the truncated 34 kDa protein of M. paratuberculosis in ELISA after pre-absorption of serum samples with the truncated34kDa protein of M. avium. All the serum samples from cattle confirmed to be infected with M. paratuberculosis were positive and those from healthy cattle were negative in the present ELISA system. These results indicate that the established ELISA detects antibodies specific to M. paratuberculosis with high specificity and sensitivity and is an useful tool for the screening of Johne’s disease.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/16905
Appears in Collections:Japanese Journal of Veterinary Research > Volume 54, Number 2-3

Submitter: 獣医学部図書室

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