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Volume 54, Number 2-3 >

Development of ELISA to detect antibodies specific to Mycobacterium aviumsubsp. paratuberculosis with truncated34kDa proteins

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.54.2-3.99

タイトル: Development of ELISA to detect antibodies specific to Mycobacterium aviumsubsp. paratuberculosis with truncated34kDa proteins
著者: Malamo, Mumeka 著作を一覧する
Sakoda, Yoshihiro 著作を一覧する
Ozaki, Hiroichi 著作を一覧する
Kida, Hiroshi 著作を一覧する
キーワード: cattle
diagnosis
ELISA
mycobacterium
paratuberculosis
発行日: 2006年11月30日
出版者: The Graduate School of Veterinary Medicine, Hokkaido University
誌名: Japanese Journal of Veterinary Research
巻: 54
号: 2-3
開始ページ: 99
終了ページ: 107
抄録: To develop ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis ),the carboxyl termini of the 34 kDa proteins of M. paratuberculosis and Mycobacterium avium subsp. avium(M. avium)were expressed in Escherichia coli expression system. Antibodies specific to M. paratuberculosis were detected with the truncated 34 kDa protein of M. paratuberculosis in ELISA after pre-absorption of serum samples with the truncated34kDa protein of M. avium. All the serum samples from cattle confirmed to be infected with M. paratuberculosis were positive and those from healthy cattle were negative in the present ELISA system. These results indicate that the established ELISA detects antibodies specific to M. paratuberculosis with high specificity and sensitivity and is an useful tool for the screening of Johne’s disease.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/16905
出現コレクション:Volume 54, Number 2-3

 

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