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Microenvironment and Effect of Energy Depletion in the Nucleus Analyzed by Mobility of Multiple Oligomeric EGFPs

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Title: Microenvironment and Effect of Energy Depletion in the Nucleus Analyzed by Mobility of Multiple Oligomeric EGFPs
Authors: Pack, Changi Browse this author
Saito, Kenta Browse this author
Tamura, Mamoru Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Keywords: fluorescence correlation spectroscopy
tandem EGFP
molecular ruler
nuclear and nucleolar microenvironment
Issue Date: Nov-2006
Publisher: The Biophysical Society
Journal Title: Biophysical Journal
Volume: 91
Issue: 10
Start Page: 3921
End Page: 3936
Publisher DOI: 10.1529/biophysj.105.079467
Abstract: Four different tandem EGFPs were constructed to elucidate the nuclear microenvironment by quantifying its diffusional properties in both aqueous solution and the nuclei of living cells. Diffusion of tandem EGFP was dependent on the length of the protein as a rod-like molecule or molecular ruler in solution. On the other hand, we found two kinds of mobility, fast diffusional mobility and much slower diffusional mobility depending on cellular compartments in living cells. Diffusion in the cytoplasm and the nucleoplasm was mainly measured as fast diffusional mobility. In contrast, diffusion in the nucleolus was complex and mainly much slower diffusional mobility, although both the fast and the slow diffusional mobilities were dependent on the protein length. Interestingly, we found that diffusion in the nucleolus was clearly changed by energy depletion, even though the diffusion in the cytoplasm and the nucleoplasm was not changed. Our results suggest that the nucleolar microenvironment is sensitive to energy depletion and very different from the nucleoplasm.
Type: article (author version)
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金城 政孝

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