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Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

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Title: Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera
Authors: Kishimura, Hideki Browse this author →KAKEN DB
Hayashi, Kenji Browse this author
Keywords: Asterina pectinifera
Isolation
Marine invertebrate
Phosphatidylcholine
Phospholipase A2
Polar group specificity
Pyloric cecum
Starfish
Issue Date: Dec-1999
Publisher: Elsevier Science Inc.
Journal Title: Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Volume: 124
Issue: 4
Start Page: 483
End Page: 488
Publisher DOI: 10.1016/S0305-0491(99)00142-X
Abstract: Phospholipase A2 was purified from the pyloric ceca of the starfish Asterina pectinifera. The final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 20,000. The optimum pH and temperature of the enzyme were at around pH 9.0 and 50°C, respectively, and the activity was enhanced by sodium deoxycholate and 1 mM or higher concentration of Ca2+. The enzyme had no fatty acid specificity. Starfish phospholipase A2 hydrolyzed phosphatidylcholine more effectively than phosphatidylethanolamine.
Relation: http://www.sciencedirect.com/science/journal/10964959
Type: article (author version)
URI: http://hdl.handle.net/2115/16941
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

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