Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

Kishimura, Hideki; Hayashi, Kenji

doi:10.1016/S0305-0491(99)00142-X


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Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/16941

Title:	Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera
Authors:	Kishimura, Hideki Browse this author →KAKEN DB
Authors:	Hayashi, Kenji Browse this author
Keywords:	Asterina pectinifera
	Isolation
	Marine invertebrate
	Phosphatidylcholine
	Phospholipase A2
	Polar group specificity
	Pyloric cecum
	Starfish
Issue Date:	Dec-1999
Publisher:	Elsevier Science Inc.
Journal Title:	Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Volume:	124
Issue:	4
Start Page:	483
End Page:	488
Publisher DOI:	10.1016/S0305-0491(99)00142-X
Abstract:	Phospholipase A2 was purified from the pyloric ceca of the starfish Asterina pectinifera. The final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 20,000. The optimum pH and temperature of the enzyme were at around pH 9.0 and 50°C, respectively, and the activity was enhanced by sodium deoxycholate and 1 mM or higher concentration of Ca2+. The enzyme had no fatty acid specificity. Starfish phospholipase A2 hydrolyzed phosphatidylcholine more effectively than phosphatidylethanolamine.
Relation:	http://www.sciencedirect.com/science/journal/10964959
Type:	article (author version)
URI:	http://hdl.handle.net/2115/16941
Appears in Collections:	水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村栄毅

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