Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera

Kishimura, Hideki; Hayashi, Kenji

doi:10.1016/S1096-4959(02)00062-3


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Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera

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Title:	Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera
Authors:	Kishimura, Hideki Browse this author →KAKEN DB
Authors:	Hayashi, Kenji Browse this author
Keywords:	Asterina pectinifera
	Calcium ion
	Isolation
	Marine invertebrate
	pH stability
	Pyloric cecum
	Starfish
	Trypsin
Issue Date:	Jun-2002
Publisher:	Elsevier Science Inc.
Journal Title:	Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Volume:	132
Issue:	2
Start Page:	485
End Page:	490
Publisher DOI:	10.1016/S1096-4959(02)00062-3
Abstract:	Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28 000. Optimum pH and temperature of A. pectinifera trypsin for hydrolysis of Nα-p-Tosyl--arginine methyl ester hydrochloride were approximately pH 8.0 and 55 °C, respectively. A. pectinifera trypsin was unstable at above 50 °C and below pH 5.0, and was not activated by adding Ca2+. The N-terminal amino acid sequence of A. pectinifera trypsin, IVGGHEF, was found.
Relation:	http://www.sciencedirect.com/science/journal/10964959
Type:	article (author version)
URI:	http://hdl.handle.net/2115/16943
Appears in Collections:	水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村栄毅

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