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Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera

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Title: Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera
Authors: Kishimura, Hideki Browse this author →KAKEN DB
Hayashi, Kenji Browse this author
Keywords: Asterina pectinifera
Calcium ion
Isolation
Marine invertebrate
pH stability
Pyloric cecum
Starfish
Trypsin
Issue Date: Jun-2002
Publisher: Elsevier Science Inc.
Journal Title: Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology
Volume: 132
Issue: 2
Start Page: 485
End Page: 490
Publisher DOI: 10.1016/S1096-4959(02)00062-3
Abstract: Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28 000. Optimum pH and temperature of A. pectinifera trypsin for hydrolysis of Nα-p-Tosyl--arginine methyl ester hydrochloride were approximately pH 8.0 and 55 °C, respectively. A. pectinifera trypsin was unstable at above 50 °C and below pH 5.0, and was not activated by adding Ca2+. The N-terminal amino acid sequence of A. pectinifera trypsin, IVGGHEF, was found.
Relation: http://www.sciencedirect.com/science/journal/10964959
Type: article (author version)
URI: http://hdl.handle.net/2115/16943
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

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