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Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.

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Title: Lipid raft disruption prevents apoptosis induced by 2-chloro-2'-deoxyadenosine (Cladribine) in leukemia cell lines.
Authors: Takahashi, Eriko Browse this author
Inanami, Osamu Browse this author →KAKEN DB
Ohta, Toshio Browse this author →KAKEN DB
Matsuda, Akira Browse this author →KAKEN DB
Kuwabara, Mikinori Browse this author
Keywords: Apoptosis
Leukemia
2-Chloro-2'-deoxyadenosine
Lipid rafts
Calcium
L-type Ca2+ channel
Issue Date: Dec-2006
Publisher: Elsevier Ltd.
Journal Title: Leukemia Research
Volume: 30
Issue: 12
Start Page: 1555
End Page: 1561
Publisher DOI: 10.1016/j.leukres.2006.04.008
PMID: 16730061
Abstract: To clarify the role of lipid rafts in 2-chloro-2′-deoxyadenosine (2CdA; Cladribine)-induced apoptosis, the effects of disruption of lipid rafts by methyl-β-cyclodextrin (MβCD) and filipin on 2CdA-induced apoptosis were investigated in four human acute lymphoblastic leukemia (ALL) cell lines comprised of T cells (MOLT-4, Jurkat) and B cells (NALM, BALL-1). The disruption of lipid rafts significantly inhibited 2CdA-induced apoptosis, indicating the crucial role of lipid rafts in the induction of apoptosis in leukemia cells. These reagents significantly inhibited 2CdA-induced elevation of the intracellular calcium concentration ([Ca2+]i) in MOLT-4 cells, and 2CdA-induced apoptosis was partly inhibited by the Ca2+ chelators BAPTA-AM and EGTA, and the L-type Ca2+ channel blocker nifedipine. On the other hand, they had no effects on the cellular uptake of 2CdA. These results indicated that lipid rafts partly contributed to 2CdA-induced apoptosis by regulating Ca2+ influx via the plasma membrane.
Relation: http://www.sciencedirect.com/science/journal/01452126
Type: article (author version)
URI: http://hdl.handle.net/2115/16953
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 桑原幹典

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