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Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori

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Title: Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori
Authors: Takemura, Yoko Browse this author
Sahara, Ken Browse this author →KAKEN DB
Mochida, Yuji Browse this author
Ohnuma, Akio Browse this author
Keywords: Apyrene sperm
Artificial insemination
Eupyrene sperm
Issue Date: Oct-2006
Publisher: Elsevier Ltd.
Journal Title: Journal of Insect Physiology
Volume: 52
Issue: 10
Start Page: 1021
End Page: 1026
Publisher DOI: 10.1016/j.jinsphys.2006.06.010
PMID: 16963075
Abstract: Female moths of Bombyx mori were artificially inseminated with cryopreserved semen. The fertility of inseminated females varied from 0% to 76.9% depending on the strain. Addition of fresh semen from triploid males, which are infertile but whose semen includes intact apyrene sperm, greatly improved fecundity of cryopreserved semen from normal males. Frozen apyrene sperm from the triploid donors also improved the fecundity of females, inseminated with cryopreserved normal semen, but less than fresh semen from triploid males. Fertilization success in B. mori requires the presence of both, intact eupyrene and apyrene sperm. Our results show that eupyrene sperm tolerate the cryopreservation process better than apyrene sperm. Hence, we recommend to add apyrene sperm from the triploid donors as helper sperm routinely to cryopreserved semen in artificial insemination. This may advance the application of cryopreservation as a routine technique to maintain silkworm resources. The technique may also be applicable to other moth and butterfly species which, like B. mori, possess eupyrene and apyrene sperm.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐原 健

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