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The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94Å resolution reveals a possible open form with a wider active-site cleft

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Title: The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94Å resolution reveals a possible open form with a wider active-site cleft
Authors: Gao, Yong-Gui Browse this author
Yao, Min Browse this author →KAKEN DB
Okada, Ayuko Browse this author
Tanaka, Isao Browse this author →KAKEN DB
Keywords: 2'-5' RNA ligase
2H phosphoesterase superfamily
Pyrococcus horikoshii
Issue Date: 1-Dec-2006
Publisher: Blackwell Publishing
Journal Title: Acta Crystallographica Section F Structural Biology and Crystallization Communications
Volume: 62
Issue: 12
Start Page: 1196
End Page: 1200
Publisher DOI: 10.1107/S1744309106046616
PMID: 17142895
Abstract: Bacterial and archaeal 2'-5' RNA ligases, members of the 2H phosphoesterase superfamily, catalyze the linkage of the 5' and 3' exons via a 2'-5'-phosphodiester bond during tRNA-precursor splicing. The crystal structure of the 2'-5' RNA ligase PH0099 from Pyrococcus horikoshii OT3 was solved at 1.94 Å resolution (PDB code 1vgj). The molecule has a bilobal α+β arrangement with two antiparallel β-sheets constituting a V-shaped active-site cleft, as found in other members of the 2H phosphoesterase superfamily. The present structure was significantly different from that determined previously at 2.4 Å resolution (PDB code 1vdx) in the active-site cleft; the entrance to the cleft is wider and the active site is easily accessible to the substrate (RNA precursor) in our structure. Structural comparison with the 2'-5' RNA ligase from Thermus thermophilus HB8 also revealed differences in the RNA precursor-binding region. The structural differences in the active-site residues (tetrapeptide motifs H-X-T/S-X) between the members of the 2H phosphoesterase superfamily are discussed.
Relation: http://journals.iucr.org/
Type: article
URI: http://hdl.handle.net/2115/17087
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 姚 閔

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