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Hokkaido University Collection of Scholarly and Academic Papers >
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The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94Å resolution reveals a possible open form with a wider active-site cleft
| Title: | The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94Å resolution reveals a possible open form with a wider active-site cleft |
| Authors: | Gao, Yong-Gui Browse this author | | Yao, Min Browse this author →KAKEN DB | | Okada, Ayuko Browse this author | | Tanaka, Isao Browse this author →KAKEN DB |
| Keywords: | 2'-5' RNA ligase | | 2H phosphoesterase superfamily | | Pyrococcus horikoshii |
| Issue Date: | 1-Dec-2006 |
| Publisher: | Blackwell Publishing |
| Journal Title: | Acta Crystallographica Section F Structural Biology and Crystallization Communications |
| Volume: | 62 |
| Issue: | 12 |
| Start Page: | 1196 |
| End Page: | 1200 |
| Publisher DOI: | 10.1107/S1744309106046616 |
| PMID: | 17142895 |
| Abstract: | Bacterial and archaeal 2'-5' RNA ligases, members of the 2H phosphoesterase superfamily, catalyze the linkage of the 5' and 3' exons via a 2'-5'-phosphodiester bond during tRNA-precursor splicing. The crystal structure of the 2'-5' RNA ligase PH0099 from Pyrococcus horikoshii OT3 was solved at 1.94 Å resolution (PDB code 1vgj). The molecule has a bilobal α+β arrangement with two antiparallel β-sheets constituting a V-shaped active-site cleft, as found in other members of the 2H phosphoesterase superfamily. The present structure was significantly different from that determined previously at 2.4 Å resolution (PDB code 1vdx) in the active-site cleft; the entrance to the cleft is wider and the active site is easily accessible to the substrate (RNA precursor) in our structure. Structural comparison with the 2'-5' RNA ligase from Thermus thermophilus HB8 also revealed differences in the RNA precursor-binding region. The structural differences in the active-site residues (tetrapeptide motifs H-X-T/S-X) between the members of the 2H phosphoesterase superfamily are discussed. |
| Relation: | http://journals.iucr.org/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/17087 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 姚 閔
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