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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections.

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Title: Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections.
Authors: Yamamoto, Tsuneyuki Browse this author →KAKEN DB
Domon, Takanori Browse this author →KAKEN DB
Takahashi, Shigeru Browse this author →KAKEN DB
Anjuman, Khan Ara Yasmin Browse this author
Fukushima, Chifumi Browse this author
Wakita, Minoru Browse this author
Keywords: Acellular cementum
Fresh-frozen sections
Matrix vesicles
Tissue non-specific alkaline phosphatase
Issue Date: Mar-2007
Publisher: Springer
Journal Title: Histochemistry and Cell Biology
Volume: 127
Issue: 3
Start Page: 303
End Page: 311
Publisher DOI: 10.1007/s00418-006-0242-x
PMID: 17043865
Abstract: This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis (2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP- and OPN-immunoreactive, initial cementum layer appeared. At this point, cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: during the initial cementogenesis, cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment, mineralization advances along collagen fibrils without matrix vesicles.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山本 恒之

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