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Chemokines in bronchiolar epithelium in the development of chronic obstructive pulmonary disease.

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タイトル: Chemokines in bronchiolar epithelium in the development of chronic obstructive pulmonary disease.
著者: Fuke, Satoshi 著作を一覧する
Betsuyaku, Tomoko 著作を一覧する
Nasuhara, Yasuyuki 著作を一覧する
Morikawa, Toshiaki 著作を一覧する
Katoh, Hiroyuki 著作を一覧する
Nishimura, Masaharu 著作を一覧する
発行日: 2004年10月
出版者: American Thoracic Society
誌名: American Journal of Respiratory Cell and Molecular Biology
巻: 31
号: 4
開始ページ: 405
終了ページ: 412
出版社 DOI: 10.1165/rcmb.2004-0131OC
抄録: The inflammatory chemokines interleukin-8, macrophage inflammatory protein-1, and monocyte chemoattractant protein-1, are reportedly involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Although bronchiolar epithelial cells and macrophages are known to be the cellular sources, the relative contribution of each cell type remains to be elucidated. In the present study, we first quantified cytokine mRNA in human bronchiolar epithelial cells and macrophages obtained using laser-capture microdissection and explored the relationship with early-stage COPD. Only in bronchiolar epithelial cells were interleukin-8, macrophage inflammatory protein-1, and monocyte chemoattractant protein-1 mRNA levels higher in smokers with airflow limitation and/or emphysema than those in never-smokers or smokers without either airflow limitation or emphysema. No difference was observed in macrophages. Complementary DNA (cDNA) array further revealed the overexpression of CC chemokine receptor 2 in bronchiolar epithelial cells from smokers with airflow limitation and/or emphysema. This study supports the role of bronchiolar epithelium as the source of increased inflammatory chemokine levels in the early development of COPD and also demonstrates the potential use of laser-capture microdissection, combined with reverse transcriptase–polymerase chain reaction and cDNA microarrays, to investigate functional profiles of individual structural and inflammatory cells in human lungs.
Relation (URI): http://ajrcmb.atsjournals.org/
資料タイプ: article
URI: http://hdl.handle.net/2115/17090
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 別役 智子

 

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