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ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies

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Title: ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies
Authors: Nagano, Masashi Browse this author →KAKEN DB
Katagiri, Seiji Browse this author →KAKEN DB
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Keywords: ATP
Developmental ability
Maturation
Mitochondria
Oocyte morphology
Issue Date: Nov-2006
Publisher: Cambridge University Press
Journal Title: Zygote
Volume: 14
Issue: 4
Start Page: 299
End Page: 304
Publisher DOI: 10.1017/S0967199406003807
PMID: 17266788
Abstract: We examined the relationship among morphological appearance (six groups) of bovine oocytes, ATP content and maturational/developmental ability. Oocytes with a brown ooplasm (with or without a dark region) had intermediate levels of ATP at the germinal vesicle (GV) stage and showed higher rates of first polar body (PB) extrusion than the other groups. Oocytes with a low level of ATP (oocytes with a pale ooplasm without dark clusters) and oocytes with a high level of ATP (oocytes with a black ooplasm) showed lower rates of PB extrusion. During in vitro maturation, ATP levels in oocytes decreased at around GV breakdown and increased toward metaphase II (MII). MII oocytes having a brown ooplasm with a dark region, which had good developmental capacity, had a relatively high level of ATP. MII oocytes with a brown or pale ooplasm without dark clusters, which had poor developmental capacity, had low ATP levels. MII oocytes with a black ooplasm, which had poor developmental capacity, had an unusually high level of ATP. These results suggest that the morphological appearance of bovine oocytes is closely related to their ATP levels and that cytoplasmic morphology will give an advantage for the selection of oocytes with a high maturational and developmental ability.
Rights: Copyright © 2006 Cambridge University Press
Type: article
URI: http://hdl.handle.net/2115/17148
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 芳幸

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