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Cellular expression of Noc2, a Rab effector protein, in endocrine and exocrine tissues in the mouse.

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タイトル: Cellular expression of Noc2, a Rab effector protein, in endocrine and exocrine tissues in the mouse.
著者: Teramae, Hiroki 著作を一覧する
Fujimoto, Wakako 著作を一覧する
Seino, Susumu 著作を一覧する
Iwanaga, Toshihiko 著作を一覧する
キーワード: Rab
Endocrine cell
Exocrine cell
発行日: 2007年 1月
出版者: Springer
誌名: Histochemistry and Cell Biology
巻: 127
号: 1
開始ページ: 1
終了ページ: 11
出版社 DOI: 10.1007/s00418-006-0207-0
抄録: Noc2 is a Rab effector which participates in regulated exocytosis. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues. Noc2-deficient mice, however, exhibit marked accumulation of secretory granules in exocrine cells rather than endocrine cells. In the present study, we investigated localization of Noc2 immunohistochemically in various endocrine and exocrine tissues in normal mice. Western blotting detected a Noc2-immunoreactive band of 38 kDa in isolated pancreatic islets, the adrenal gland, pituitary gland, and thyroid gland. Immunostaining for Noc2 labeled endocrine cells in the adrenal medulla and adenohypophysis, pancreatic islet cells, thyroid parafollicular cells, and gut endocrine cells, supporting the notion that Noc2 is a Rab effector protein shared by amine/peptide-secreting endocrine cells. Besides endocrine tissues, granular ducts in salivary glands contained Noc2. Although immunostaining failed to detect Noc2 in acinar cells of all exocrine glands examined, reverse transcriptase-polymerase chain reaction analysis detected the mRNA expression in exocrine pancreas. Ultrastructurally, Noc2 immunoreactivity was associated with the limiting membrane of granules in both pancreatic endocrine and salivary duct exocrine cells. The cellular and subcellular localizations of Noc2 should yield key information on its functional significance as well as account for the phenotype in Noc2-deficient mice.
Rights: The original publication is available at
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 岩永 敏彦


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