HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
医学研究院・医学院  >
雑誌発表論文等  >

Extrasynaptic localization of GABA in the developing mouse cerebellum

NR50-4.pdf3.36 MBPDF見る/開く

タイトル: Extrasynaptic localization of GABA in the developing mouse cerebellum
著者: Takayama, Chitoshi 著作を一覧する
Inoue, Yoshiro 著作を一覧する
キーワード: Vesicular GABA transporter
GABAA receptor
Purkinje cell
Golgi cell
GABAergic synapse
発行日: 2004年12月
出版者: Elsevier Ireland Ltd
誌名: Neuroscience Research
巻: 50
号: 4
開始ページ: 447
終了ページ: 458
出版社 DOI: 10.1016/j.neures.2004.08.012
抄録: In the adult brain, γ-amino butyric acid (GABA) is synaptically released and mediates inhibitory transmission. Recent studies have revealed that GABA is a trophic factor for brain development. To reveal the distribution of GABA and its secretion mechanisms during brain development, we investigated the immunohistochemical localization of two molecules, GABA and vesicular GABA transporter (VGAT), which is a GABAergic vesicle protein, in the developing mouse cerebellum by means of newly developed antibodies. Furthermore, we tested the relationship between developmental changes in distribution of above two molecules in the presynapses and ontogeny of GABAergic synapses. GABAergic synapses were detected by immunohistochemistry for the GABAA receptor α1 subunit, which is an essential subunit for inhibitory synaptic transmission in the mature cerebellar cortex. Until postnatal day 7 (P7), GABA was localized throughout the GABAergic neurons, and VGAT accumulated at axon varicosities and growth cones, where the α1 subunit did not accumulate. After P10, both GABA and VGAT became confined to the terminal sites where the α1 subunit was localized. These results suggested that GABA was extrasynaptically released from axon varicosities and growth cones by vesicular secretion ‘exocytosis’ and from all parts of GABAergic neurons during the cerebellar development by non-vesicular secretion ‘diacrine’.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高山 千利


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学