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Extrasynaptic localization of GABA in the developing mouse cerebellum

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Title: Extrasynaptic localization of GABA in the developing mouse cerebellum
Authors: Takayama, Chitoshi Browse this author →KAKEN DB
Inoue, Yoshiro Browse this author →KAKEN DB
Keywords: Vesicular GABA transporter
GABAA receptor
Purkinje cell
Golgi cell
GABAergic synapse
Issue Date: Dec-2004
Publisher: Elsevier Ireland Ltd
Journal Title: Neuroscience Research
Volume: 50
Issue: 4
Start Page: 447
End Page: 458
Publisher DOI: 10.1016/j.neures.2004.08.012
PMID: 15567482
Abstract: In the adult brain, γ-amino butyric acid (GABA) is synaptically released and mediates inhibitory transmission. Recent studies have revealed that GABA is a trophic factor for brain development. To reveal the distribution of GABA and its secretion mechanisms during brain development, we investigated the immunohistochemical localization of two molecules, GABA and vesicular GABA transporter (VGAT), which is a GABAergic vesicle protein, in the developing mouse cerebellum by means of newly developed antibodies. Furthermore, we tested the relationship between developmental changes in distribution of above two molecules in the presynapses and ontogeny of GABAergic synapses. GABAergic synapses were detected by immunohistochemistry for the GABAA receptor α1 subunit, which is an essential subunit for inhibitory synaptic transmission in the mature cerebellar cortex. Until postnatal day 7 (P7), GABA was localized throughout the GABAergic neurons, and VGAT accumulated at axon varicosities and growth cones, where the α1 subunit did not accumulate. After P10, both GABA and VGAT became confined to the terminal sites where the α1 subunit was localized. These results suggested that GABA was extrasynaptically released from axon varicosities and growth cones by vesicular secretion ‘exocytosis’ and from all parts of GABAergic neurons during the cerebellar development by non-vesicular secretion ‘diacrine’.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高山 千利

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