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Morphological development and maturation of the GABAergic synapses in the mouse cerebellar granular layer

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Title: Morphological development and maturation of the GABAergic synapses in the mouse cerebellar granular layer
Authors: Takayama, Chitoshi Browse this author →KAKEN DB
Inoue, Yoshiro Browse this author →KAKEN DB
Keywords: Synapse formation
Glutamic acid decarboxylase
GABAA receptor
Synaptic glomerulus
Golgi cell
Granule cell
Issue Date: 21-Jun-2004
Publisher: Elsevier B.V.
Journal Title: Developmental Brain Research
Volume: 150
Issue: 2
Start Page: 177
End Page: 190
Publisher DOI: 10.1016/j.devbrainres.2004.03.011
PMID: 15158081
Abstract: In the adult central nervous system (CNS), γ-amino butyric acid (GABA) is a predominant inhibitory neurotransmitter, which regulates glutamatergic activity. Recent studies have revealed that GABA serves as an excitatory transmitter in the immature CNS, and is involved in brain morphogenesis. To elucidate how GABA exerts its effect on immature neurons and how GABAergic synapses are formed, we examined both development of pre- and post-synaptic elements of the GABAergic synapses formed between granule and Golgi cells in the mouse cerebellar granular layer. Immunohistochemistry for glutamic acid decarboxylase (GAD) demonstrated that GABA was localized throughout the Golgi cells before postnatal day 7 (P7), and became confined to the axon terminals during the second postnatal week. Electron microscopic analysis demonstrated that GABAergic synapses were clearly detected at P10. In situ hybridization and immunohistochemistry for the GABAA receptor α1 and α6 subunits, which are mainly involved in inhibitory synaptic transmission, demonstrated that both subunits appeared at P7. Distribution of both subunits expanded in the granular layer with special reference to the development of GABAergic synapses. Furthermore, the majority of the subunits accumulated adjacent to the GABAergic terminals. These results suggested that in the granular layer, GABA might be non-synaptically secreted from Golgi cell axons and dendrites during the first postnatal week. From the second postnatal week, GABA is synaptically released and begins to mediate inhibitory transmission. Furthermore, it was suggested that GABAergic innervation could initiate expression and trafficking of the GABAA receptors containing the α1 and α6 subunits.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高山 千利

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