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Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins.

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この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/17169

タイトル: Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins.
著者: Matsuo, Kouki 著作を一覧する
Hong, Jin- Sung 著作を一覧する
Tabayashi, Noriko 著作を一覧する
Ito, Akira 著作を一覧する
Masuta, Chikara 著作を一覧する
Matsumura, Takeshi 著作を一覧する
キーワード: Acidic fibroblast growth factor
Cucumber mosaic virus
Modifiable vector
Transient expression
Virus vector
発行日: 2007年 1月
出版者: Springer
誌名: Planta
巻: 225
号: 2
開始ページ: 277
終了ページ: 286
出版社 DOI: 10.1007/s00425-006-0346-5
抄録: We have developed Cucumber mosaic virus (CMV) as a plant virus vector especially for production of pharmaceutical proteins. The CMV vector is a vector modifiable for different host plants and does not require further engineering steps. CMV contains three genomic RNA molecules (RNAs 1–3) necessary for infectivity. With this system, instead of creating different vector constructs for each plant we use, we take advantage of the formation of pseudrecombinants between two CMV isolates by simply reassembling a vector construct (RNA 2 base) and an RNA molecule containing the host determinant (mostly RNA 3). In this study, the gene for acidic fibroblast growth factor (aFGF), one of the human cytokines, was cloned under the control of the subgenomic promoter for RNA 4A of the CMV-based vector, C2-H1. Infected Nicotiana benthamiana plants produced aFGF at levels up to 5–8% of the total soluble protein. The tobacco-produced aFGF was purified, and its biological activity was confirmed. Using this system, which provides a versatile and viable strategy for the production of therapeutic proteins in plants, we also demonstrated a high level of aFGF in Glycine max (soybean) and Arabidopsis thaliana.
記述: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/17169
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 増田 税

 

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