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Suppressive Effect of Modified Dominant Negative RAS Mutant on Human Cancer by Gene Transfer with Non-Viral Vector

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Title: Suppressive Effect of Modified Dominant Negative RAS Mutant on Human Cancer by Gene Transfer with Non-Viral Vector
Authors: Suzuki, H. Browse this author
Kuzumaki, S. Browse this author
Nakagawa, K. Browse this author
Takimoto, M. Browse this author
Shichinohe, T. Browse this author →KAKEN DB
Keywords: H-Ras
Dominant negative mutant
Human cancer
Gene therapy
Adenovirus vector
Non-viral vector
HVJ envelope
Issue Date: Nov-2006
Publisher: Landes Bioscience
Journal Title: Cancer Biology & Therapy
Volume: 5
Issue: 11
Start Page: 1487
End Page: 1491
PMID: 17102594
Abstract: N116Y, H-RAS mutant, has dominant negative activity in the RAS function and a suppressive effect on the growth of various types of cancer cells. However, a replication error of N116Y is of potential concern for carcinogenesis in clinical application. To decease the concern, we constructed modified N116Y by deleting the carboxyl terminus, which is necessary for the oncogenic function of Ras. One of the C-terminal deletion mutants of N116Y, N116Y-Cdel2 showed a growth-suppressing effect on various human cancer cell lines in vitro: the cervical cancer cell line HeLa, the pancreatic cancer cell line PCI43, the colon cancer cell lines SW480 and LoVo, and the tongue cancer cell line SAS. In addition, the suppressive effect of N116Y-Cdel2 on LoVo cells was also observed in vivo using a non-viral gene transfer vector, HVJ envelope. Our experiments suggest that the modified N116Y is a potential candidate gene for human cancer gene therapy.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 七戸 俊明

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