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Volume 15, Number 2 >

STUDIES ON ECHINOCOCCOSIS XIX : HEAT STABLE ANTIGEN IN CYSTIC FLUID OF ECHINOCOCCUS MULTILOCULARIS

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.15.2.85

Title: STUDIES ON ECHINOCOCCOSIS XIX : HEAT STABLE ANTIGEN IN CYSTIC FLUID OF ECHINOCOCCUS MULTILOCULARIS
Authors: ORIHARA, Miyoji Browse this author
Issue Date: Jun-1967
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 15
Issue: 2
Start Page: 85
End Page: 97
Abstract: For inactivation of antigenicity of host components in cystic fluid of E. multilocularis, heat treatment experiments were conducted. Fractions which are reactive to serum from infected mice with E. multilocularis were collected by gel filtration of Sephadex G 75,and were heated at 100℃ for 15 minutes. For a comparative study, mild heating was also performed. For serological tests, the CF test and gel diffusion test were used. Experimental results obtained may be summarized as follows : 1) Reactive components of host origin in cystic fluid are partially lost at 70℃ for 20 minutes and are almost destroyed at 100℃ for 15 minutes. 2) In the gel diffusion test, most antigens originated from the parasite in cystic fluid are stable at 100℃ for 15 minutes in test with serum from mice with E. multilocularis, and these precipitates are stained strongly with PAS. So, polysaccharides seem to take a part in these precipitates. In antigens originated from the parasite, heat labile antigens are also present while it is not often reactive in test with sera from mice. 3) In the CF test, heated fractionated Emc titer is low, in comparison with raw fractionated Emc in test with sera from human beings and infected mice.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/1870
Appears in Collections:Japanese Journal of Veterinary Research > Volume 15, Number 2

Submitter: 獣医学部図書室

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