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Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)

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Title: Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)
Authors: Nakai, Hiroyuki Browse this author
Ito, Tatsuya Browse this author
Hayashi, Masatoshi Browse this author
Kamiya, Koutarou Browse this author
Yamamoto, Takeshi Browse this author
Matsubara, Kazuki Browse this author
Kim, Young-Min Browse this author
Jintanart, Wongchawalit Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Mori, Haruhide Browse this author
Chiba, Seiya Browse this author
Sanoa, Yoshio Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: α-Glucosidase isoforms and isozymes
Post-translational proteolysis
Starch granules degradation
α-Glucosidase multigene family
Alternative splicing
Issue Date: Jan-2007
Publisher: Elsevier Masson SAS
Journal Title: Biochimie
Volume: 89
Issue: 1
Start Page: 49
End Page: 62
Publisher DOI: 10.1016/j.biochi.2006.09.014
PMID: 17056172
Abstract: Two isoforms of α-glucosidases (ONG2-I and ONG2-II) were purified from dry rice seeds (Oryza sativa L., var Nipponbare). Both ONG2-I and ONG2-II were the gene products of ONG2 mRNA expressed in ripening seeds. Each enzyme consisted of two components of 6 kDa-peptide and 88 kDa-peptide encoded by this order in ONG2 cDNA (ong2), and generated by post-translational proteolysis. The 88 kDa-peptide of ONG2-II had 10 additional N-terminal amino acids compared with the 88 kDa-peptide of ONG2-I. The peptides between 6 kDa and 88 kDa components (26 amino acids for ONG2-I and 16 for ONG2-II) were removed by post-translational proteolysis. Proteolysis induced changes in adsorption and degradation of insoluble starch granules. We also obtained three α-glucosidase cDNAs (ong1, ong3, and ong4) from ripening seeds. The ONG1, ONG2, and ONG4 genes were situated in distinct locus of rice genome. The transcripts encoding ONG2 and ONG3 were generated by alternative splicing. Members of α-glucosidase multigene family are differentially expressed during ripening and germinating stages in rice.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

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