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Volume 18, Number 4 >

HEMATOLOGICAL OBSERVATIONS OF LEUKOCYTES COLLECTED BY WATER METHOD FROM BOVINE PERIPHERAL BLOOD

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.18.4.173

Title: HEMATOLOGICAL OBSERVATIONS OF LEUKOCYTES COLLECTED BY WATER METHOD FROM BOVINE PERIPHERAL BLOOD
Authors: ODAJIMA, Mikiyo Browse this author
SONODA, Mitsuo Browse this author
Issue Date: Dec-1970
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 18
Issue: 4
Start Page: 173
End Page: 179
Abstract: An attempt was made to collect a large number of leukocytes without red cells from bovine peripheral blood by the use of the water method improved by the authors, then they were examined hematologically. 1) In the collection of leukocytes by the water method, it was found that using blood and water, in the ratio of 1 to 1.5,with the restoration of isotonicity after 20 seconds was the most favorable condition for bovine blood. 2) In the light microscopy of the collected leukocytes smears stained with Giemsa stain, a significant decrease of normal cells and an increase of destructed cells was observed in comparison with those of the untreated control blood, respectively, but in so-called ghost cells, no significant changes were observed. 3) A significant increase of so-called dead cells was observed in the collected cells in comparison with those of the untreated control blood. 4) By the treatment with water, the phagocytic activities of carbon particles were decreased significantly in monocytes and eosinophils, but in neutrophils no significant changes were observed in comparison with those of the untreated control blood. 5) In the electron microscopy of the collected leukocytes, the defects of the fine structure of the cells were not so marked that almost all the collected cells distinguished their kind of cells by the characteristics of their fine structures. 6) In the differential count of the collected leukocytes, a significant increase of neutrophils and a decrease of lymphocytes was observed, but no significant changes were observed in monocytes, eosinophils and basophils in comparison with the untreated control blood.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/1967
Appears in Collections:Japanese Journal of Veterinary Research > Volume 18, Number 4

Submitter: 獣医学部図書室

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