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Volume 20, Number 1-2 >

PURIFICATION AND CRYSTALLIZATION OF CLOSTRIDIUM BOTULINUM TYPE C TOXIN

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.20.1-2.19

Title: PURIFICATION AND CRYSTALLIZATION OF CLOSTRIDIUM BOTULINUM TYPE C TOXIN
Authors: SYUTO, Bunei Browse this author
KUBO, Shuichiro Browse this author
Issue Date: Jun-1972
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 20
Issue: 1-2
Start Page: 19
End Page: 30
Abstract: A procedure for purification and crystallization of Clostridium botulinum type C toxin is described. The toxin was precipitated from the culture by addition of zinc chloride and eluted by dibasic sodium phosphate solution from the precipitate. The toxin was precipitated with ammonium sulfate to 34% saturation at 4℃ and the toxin solution dissolved in acetate-phosphate buffer was chromatographed by the connected columns of Sephadex G-75-DEAE cellulose-QAE Sephadex A-50 at pH 7.0. The eluate had a specific toxicity of 3.8×10^7 MLD/1.0 E_<277.5mμ>. The toxin was crystallized from the solution dialysed against the saturated ammonium sulfate solution by ultrafiltration. The crystalline toxin had a specific toxicity of 3.0×10^7 MLD/1.0 E_<277.5mμ> and a faint hemagglutination activity. The crystalline toxin contained at least two components in acrylamide gel electrophoresis and the agar gel immunodiffusion test, respectively.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/1989
Appears in Collections:Japanese Journal of Veterinary Research > Volume 20, Number 1-2

Submitter: 獣医学部図書室

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