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Volume 27, Number 3-4 >

SOME ASPECTS OF BOVINE OVA CULTURE IN VITRO

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.27.3-4.49

Title: SOME ASPECTS OF BOVINE OVA CULTURE IN VITRO
Authors: KANAGAWA, Hiroshi Browse this author
Issue Date: 27-Dec-1979
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 27
Issue: 3-4
Start Page: 49
End Page: 54
Abstract: One hundred and thirty-three fertilized ova were grouped into three categories ; normal, young and degenerated on the 5th day after insemination. A simple culture technique of a stoppered test tube with Brinster's medium and a gas mixture was applied. Thirty-eight out of 46 normal ova (82.6%) were developed into the blastocysts. The young and degenerated ova showed very poor development. Ten normal ova were kept for 2〜4 hours at room temperature before culture and 56 normal ova were exposed under microscopic light for 30 seconds or 30 minutes before culture. Nine ova from the former group and 46 ova from the latter group were developed into the blastocysts, respectively.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2175
Appears in Collections:Japanese Journal of Veterinary Research > Volume 27, Number 3-4

Submitter: 獣医学部図書室

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