HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
獣医学研究科  >
Japanese Journal of Veterinary Research >
Volume 28, Number 1-2 >

IN VITRO CULTURE OF FROZEN AND THAWED MOUSE OVA

フルテキスト
KJ00003407905.pdf966.54 kBPDF見る/開く
この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.28.1-2.12

タイトル: IN VITRO CULTURE OF FROZEN AND THAWED MOUSE OVA
著者: KANAGAWA, Hiroshi 著作を一覧する
発行日: 1980年 5月31日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 28
号: 1-2
開始ページ: 12
終了ページ: 18
抄録: Seventy mouse ova at the 4--8-cell stages were collected at room temperature from mice treated with pregnant mare's serum gonadotrophin and human chorionic gonadotrophin. Seventy ova were divided into 5 groups, and each group was placed in a 0.5 ml plastic straw with 0.2 ml of Brinster's medium. Then, the straws were immersed into an ice bath (0℃) for 5 minutes. Next, an equal volume of 2 M dimethyl sulfoxide was added to the sample straw. The medium with ova was then seeded at -6℃ and cooled to -100℃ at a rate of 0.4 to 0.5℃ per minute using an inexpensive freeze-thaw apparatus. After reaching -100℃, the sample straws were directly immersed in liquid nitrogen at -196℃ and stored for 1-9 days. Following the storage period, the sample straw were thawed at 12-15℃ per minute from -196℃ to 0℃. The samples were then diluted with fresh medium and placed inside a petri dish at room temperature (20-25℃). The ova were moved immediately to a fresh medium 3 times, then incubated at 37℃ for 72 hours. Sixty out of the 70 ova were recovered from the straws after the freezing experiment, and 41 out of the 60 ova (68.3%) developed in vitro to the blastocyst stage within 72 hours.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2182
出現コレクション:Volume 28, Number 1-2

提供者: 獣医学部図書室

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学