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Volume 28, Number 3 >

COMPARISON OF MEMBRANE IMMUNOFLUORESCENCE AND CYTOTOXICITY TESTS FOR DETECTION OF CELL SURFACE ANTIGENS OF MAREK'S DISEASE LYMPHOMA-DERIVED CELL LINE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.28.3.67

Title: COMPARISON OF MEMBRANE IMMUNOFLUORESCENCE AND CYTOTOXICITY TESTS FOR DETECTION OF CELL SURFACE ANTIGENS OF MAREK'S DISEASE LYMPHOMA-DERIVED CELL LINE
Authors: SUZUKI, Kazuo Browse this author
MIKAMI, Takeshi Browse this author
KODAMA, Hiroshi Browse this author
ONUMA, Misao Browse this author
IZAWA, Hisao Browse this author
Issue Date: 6-Oct-1980
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 28
Issue: 3
Start Page: 67
End Page: 74
Abstract: The reactivity of an anti-MDCC-MSB 1 rabbit serum to Marek's disease tumor-associated surface antigen (MATSA) of a Marek's disease lymphomaderived cell line (MDCC-MSB 1) was compared by membrane immunofluorescence and cytotoxicity tests. Following results were obtained by both tests : 1) the similar reactivity of the antiserum in titrations, 2) the similar blocking activity patterns of the soluble antigens from MDCC-MSB 1 cells to the antiserum, and 3) the parallisms between the cytotoxic index and the frequency of positive cells for MATSA of the MDCC-MSB 1 cells treated with proteolytic enzymes. From these results, both tests appeared to be detecting a similar, if not identical, antigen. The cytotoxicity test was slightly more sensitive than the membrane immunofluorescence test.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2198
Appears in Collections:Japanese Journal of Veterinary Research > Volume 28, Number 3

Submitter: 獣医学部図書室

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