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Volume 30, Number 3-4 >

ANTIBODY ASSAY FOR JAPANESE ENCEPHALITIS VIRUS IN BOVINE SERUM BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.30.3-4.59

Title: ANTIBODY ASSAY FOR JAPANESE ENCEPHALITIS VIRUS IN BOVINE SERUM BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
Authors: MIYATA, Keiji Browse this author
UEDA, Masayuki Browse this author
HASHIMOTO, Nobuo Browse this author
Issue Date: 28-Dec-1982
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 30
Issue: 3-4
Start Page: 59
End Page: 67
Abstract: Enzyme-linked immunosorbent assay (ELISA) was applied for seroepidemiological study of Japanese encephalitis (JE) using bovine sera. ELISA antibody titers were four fold higher than neutralizing antibody titers. Minimum titer of positive ELISA antibody was set at a serum dilution of 1 : 40 to eliminate the non-specific reaction against normal mouse brain components in a test antigen. Antibody prevalence rates against JE virus in Kagoshima, an endemic area, were 26.0% in ELISA and 23.5% in the neutralization test. Meanwhile, sera from Hokkaido, a JE free area, had a prevalence rate of 4.3% by ELISA. Cross-reaction in ELISA between JE and Negishi virus was seen in bovine sera from Kagoshima and also in immune rabbit sera.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2263
Appears in Collections:Japanese Journal of Veterinary Research > Volume 30, Number 3-4

Submitter: 獣医学部図書室

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