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Volume 31, Number 3-4 >

ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AS A DIAGNOSTIC TOOL FOR GUATEMALAN ONCHOCERCIASIS USING A BOVINE FILARIA (ONCHOCERCA GUTTUROSA) ANTIGEN AND BLOOD SAMPLES COLLECTED ON FILTER PAPER

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.31.3-4.141

Title: ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AS A DIAGNOSTIC TOOL FOR GUATEMALAN ONCHOCERCIASIS USING A BOVINE FILARIA (ONCHOCERCA GUTTUROSA) ANTIGEN AND BLOOD SAMPLES COLLECTED ON FILTER PAPER
Authors: ITO, Mamoru Browse this author
LUJAN-T., Aracely Browse this author
FUKUMOTO, Shin-ichiro Browse this author
KAMIYA, Masao Browse this author
Keywords: ELISA
Onchocerca gutturosa
Onchocerca volvulus
filaria
Guatemala
Issue Date: 31-Oct-1983
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 31
Issue: 3-4
Start Page: 141
End Page: 150
Abstract: An ELISA for human onchocerciasis performed in Guatemala with a crude PBS extract of Onchocerca gutturosa and blood samples collected on filter paper showed a high sensitivity and specificity. A correlation between ELISA and IHA was obtained, and the former proved to be more sensitive than the latter. The ELISA values of blood samples collected on filter paper and of sera obtained from the same patient showed a high correlation. The linear correlation was also observed in ELISA values using two types of antigens, i. e., O.gutturosa and O.volvulus. 388 inhabitants of five coffee plantations were examined by the ELISA using O.gutturosa antigen. The results showed a positive reaction in 40(97.6%) of the 41 inhabitants with microfilariae(Mf)-positive/nodule-positive, and in 76(91.6%) out of 83 with Mf-positive/nodule-negative, but in only 17(21.8%) out of 78 with Mf-negative/nodule-negative in an endemic area, and 1(1.1%) out of 90 in a non-endemic area. Using a combination of O.gutturosa obtained from cattle and blood samples collected on filter paper, ELISA was found to be applicable for the immunodiagnosis of human onchocerciasis in Guatemala.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2302
Appears in Collections:Japanese Journal of Veterinary Research > Volume 31, Number 3-4

Submitter: 獣医学部図書室

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