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Volume 33, Number 1-2 >

HISTOCHEMICAL OBSERVATIONS OF LIPID DROPLETS IN MOUSE EMBRYOS

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.33.1-2.51

タイトル: HISTOCHEMICAL OBSERVATIONS OF LIPID DROPLETS IN MOUSE EMBRYOS
著者: HISHINUMA, Mitsugu 著作を一覧する
TAKAHASHI, Yoshiyuki 著作を一覧する
KANAGAWA, Hiroshi 著作を一覧する
キーワード: lipid droplet
mouse
embryo
発行日: 1985年 4月30日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 33
号: 1-2
開始ページ: 51
終了ページ: 57
抄録: Morphology of lipid droplets in 313 embryos of different developmental stages was examined. Embryos were obtained from spontaneously ovulated and superovulated mice of ddY strain. Sudan III was used to stain embryos so as to count the number of lipid droplets. Lipid droplets were classified into three groups according to the diameter : small (≦ 1.0μm), medium (1.1-3.0μm) and large (3.1-5.0μm). From the 2-cell stage to the expanded blastocyst, lipid droplets were found to be abundant. Numerous small lipid droplets were observed in all developmental stages. The number of medium and large lipid droplets increased with the development of embryos. The numbers of medium lipid droplets in the embryos at the 2-cell stage, 8-cell stage and expanded blastocyst were 0.5±0.4,11.2±3.3 and 60.3±3.2,respectively. Large lipid droplets were not observed in the 2-cell and 4-cell stages, but were found to be abundant in the morula and blastocyst. A sudden and significant increase in the number of medium and large lipid droplets was observed from the 8-cell stage to the morula (p<0.01). Similar increase of lipid droplets was also observed in morulae developed from 8-cell embryos in in vitro culture (p<0.01). The number of lipid droplets in expanded blastocysts was not affected by the ovulation methods.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2319
出現コレクション:Volume 33, Number 1-2

 

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