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Volume 40, Number 1 >

LATEX AGGLUTINATION TEST : A SIMPLE, RAPID AND PRACTICAL METHOD FOR BOVINE SERUM CRP DETERMINATION

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.40.1.1

Title: LATEX AGGLUTINATION TEST : A SIMPLE, RAPID AND PRACTICAL METHOD FOR BOVINE SERUM CRP DETERMINATION
Authors: SARIKAPUTI, M. Browse this author
MORIMATSU, M. Browse this author
YAMAMOTO, S. Browse this author
SYUTO, B. Browse this author
SAITO, M. Browse this author
NAIKI, M. Browse this author
Keywords: latex agglutination
C-reactive protein
bovine
acute phase protein
Issue Date: 29-May-1992
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 40
Issue: 1
Start Page: 1
End Page: 12
Abstract: A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 μm latex particles coated with affinity-purified antibody at a ratio of 20 μg/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incubation. This test is reliable, reproducible and the results correlate with those of the single radial immunodiffusion (SRID) test. The effect of low temperature storage on CRP concentration revealed a 30% degradation of CRP during 2 years storage at 4℃. The possible role of EDTA addition to prevent a decrease in serum CRP concentration by freezing and thawing is also discussed.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2358
Appears in Collections:Japanese Journal of Veterinary Research > Volume 40, Number 1

Submitter: 獣医学部図書室

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