HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 40, Number 1 >

DETECTION OF EQUINE IMMUNOGLOBULIN-SECRETING CELLS BY A PLAQUE ASSAY

Files in This Item:
KJ00002377531.pdf334.89 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.40.1.13

Title: DETECTION OF EQUINE IMMUNOGLOBULIN-SECRETING CELLS BY A PLAQUE ASSAY
Authors: GOTO, Ikuo Browse this author
KAMADA, Masanobu Browse this author
INABA, Mutsumi Browse this author
MAEDE, Yoshimitsu Browse this author →KAKEN DB
Keywords: hemolytic plaque assay
Protein A
horse
lymphocyte
Issue Date: 29-May-1992
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 40
Issue: 1
Start Page: 13
End Page: 18
Abstract: A protein A-hemolytic plaque assay was applied to detect immunoglobulin (Ig)-producing cells in horse peripheral blood, using pokeweed mitogen as a B lymphocyte activator. A maximum number of Ig-secreting cells was obtained when horse peripheral blood lymphocytes were cultured in a medium containing horse serum. The number of Ig-secreting cells in young horses (2 years old) was lower than that in adult horses (6 to 23 years old). In addition, the plaque formation was unchanged from blood samples kept at 4℃ for 24 hours, while blood samples kept for 72 hours did not yield plaques. These results indicate that the plaque assay is a reliable and useful method for detecting Ig-secreting cells in the peripheral blood of the horse.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2359
Appears in Collections:Japanese Journal of Veterinary Research > Volume 40, Number 1

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University