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Volume 40, Number 2-3  >

EFFECT OF NEUTRALIZING MONOCLONAL ANTIBODIES ON HANTAAN VIRUS INFECTION OF THE MACROPHAGE P388D1 CELL LINE

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.40.2-3.87

タイトル: EFFECT OF NEUTRALIZING MONOCLONAL ANTIBODIES ON HANTAAN VIRUS INFECTION OF THE MACROPHAGE P388D1 CELL LINE
著者: YAO, Jian-Sheng 著作を一覧する
ARIKAWA, Jiro 著作を一覧する
KARIWA, Hiroaki 著作を一覧する
YOSHIMATSU, Kumiko 著作を一覧する
TAKASHIMA, Ikuo 著作を一覧する
HASHIMOTO, Nobuo 著作を一覧する
キーワード: Hantaan virus
Neutralizing antibody
Macrophage
Monoclonal antibody
発行日: 1992年 9月30日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 40
号: 2-3
開始ページ: 87
終了ページ: 97
抄録: The effect of neutralizing monoclonal antibodies (N MAbs) on Hantaan virus infection of macrophages was investigated using P388D1 cells, a murine macrophage cell line. MAbs to the G1 protein (G1b) and the G2 protein (G2a and G2c) neutralized viral infectivity in P388D1 cells. N MAbs to G1b showed much higher neutralizing potency than those to G2a and G2c. With each N MAbs, two distinct effects were observed : neutralization of viral infectivity occurring at high concentrations and enhancement of that at low concentrations. Non-neutralizing MAbs, on the other hand, showed only enhancement of viral infectivity even at high concentrations without any inhibitory effects. The Fab fragments of N MAbs showed neither neutralizing nor enhancing activities. However, when the virus-Fab complexes were reacted with the anti-Fab antibodies, both neutralization and enhancement of viral infectivity were restored depending on the dose of Fab fragments. These results indicate that Hantaan virus infection of P388D1 cells is mediated by the Fc portion of the antibodies and neutralization is dependent on the concentration of N antibodies bound bivalently to the neutralization site on the virion.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2400
出現コレクション:Volume 40, Number 2-3

提供者: 獣医学部図書室

 

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