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Volume 40, Number 2-3  >

EFFECT OF NEUTRALIZING MONOCLONAL ANTIBODIES ON HANTAAN VIRUS INFECTION OF THE MACROPHAGE P388D1 CELL LINE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.40.2-3.87

Title: EFFECT OF NEUTRALIZING MONOCLONAL ANTIBODIES ON HANTAAN VIRUS INFECTION OF THE MACROPHAGE P388D1 CELL LINE
Authors: YAO, Jian-Sheng Browse this author
ARIKAWA, Jiro Browse this author
KARIWA, Hiroaki Browse this author
YOSHIMATSU, Kumiko Browse this author
TAKASHIMA, Ikuo Browse this author
HASHIMOTO, Nobuo Browse this author
Keywords: Hantaan virus
Neutralizing antibody
Macrophage
Monoclonal antibody
Issue Date: 30-Sep-1992
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 40
Issue: 2-3
Start Page: 87
End Page: 97
Abstract: The effect of neutralizing monoclonal antibodies (N MAbs) on Hantaan virus infection of macrophages was investigated using P388D1 cells, a murine macrophage cell line. MAbs to the G1 protein (G1b) and the G2 protein (G2a and G2c) neutralized viral infectivity in P388D1 cells. N MAbs to G1b showed much higher neutralizing potency than those to G2a and G2c. With each N MAbs, two distinct effects were observed : neutralization of viral infectivity occurring at high concentrations and enhancement of that at low concentrations. Non-neutralizing MAbs, on the other hand, showed only enhancement of viral infectivity even at high concentrations without any inhibitory effects. The Fab fragments of N MAbs showed neither neutralizing nor enhancing activities. However, when the virus-Fab complexes were reacted with the anti-Fab antibodies, both neutralization and enhancement of viral infectivity were restored depending on the dose of Fab fragments. These results indicate that Hantaan virus infection of P388D1 cells is mediated by the Fc portion of the antibodies and neutralization is dependent on the concentration of N antibodies bound bivalently to the neutralization site on the virion.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2400
Appears in Collections:Japanese Journal of Veterinary Research > Volume 40, Number 2-3

Submitter: 獣医学部図書室

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