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Volume 40, Number 4 >

DEVELOPMENT OF MOUSE EMBRYONIC NUCLEI TRANSFERRED TO ENUCLEATED OOCYTES AND ZYGOTES

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.40.4.149

Title: DEVELOPMENT OF MOUSE EMBRYONIC NUCLEI TRANSFERRED TO ENUCLEATED OOCYTES AND ZYGOTES
Authors: CHEONG, Hee Tae Browse this author
TAKAHASHI, Yoshiyuki Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: Nuclear transplantation
nuclear reprogramming
mouse
Issue Date: 28-Dec-1992
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 40
Issue: 4
Start Page: 149
End Page: 159
Abstract: The present study was conducted to examine the development of nuclear transplant embryos produced by transplanting nuclei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, four- and eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without staining. Fusion and oocyte activation were performed by means of electric fields. Similar development rates to the blastocyst stage were obtained from enucleated oocytes (28.0%) and zygotes (30.9%) reconstituted with nuclei from late two-cell embryos. Cleavage and blastocoele formation of reconstituted embryos occurred at around the same time as observed in the control embryos, with some exceptions. After transfer to recipient females, live young were obtained from both reconstituted oocytes (9.1%) and zygotes (11.5%) that received a nucleus from late two-cell embryos. The results indicate that enucleated zygotes as well as oocytes can support development to term of nuclei introduced from late two-cell embryos in which activation of the embryonic genome has occured, which may be a result of the reprogramming of the donor nucleus.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2406
Appears in Collections:Japanese Journal of Veterinary Research > Volume 40, Number 4

Submitter: 獣医学部図書室

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