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Establishment of a newly improved detection system for NF-κB activity

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/24260

Title: Establishment of a newly improved detection system for NF-κB activity
Authors: Matsuda, Mayuko Browse this author
Tsukiyama, Tadasuke Browse this author
Bohgaki, Miyuki Browse this author
Nonomura, Katsuya Browse this author
Hatakeyama, Shigetsugu Browse this author →KAKEN DB
Keywords: NF-κB
Transcription
Reporter system
Luciferase
EGFP
Issue Date: 15-Apr-2007
Publisher: Elsevier B.V.
Journal Title: Immunology Letters
Volume: 109
Issue: 2
Start Page: 175
End Page: 181
Publisher DOI: 10.1016/j.imlet.2007.02.007
PMID: 17368808
Abstract: The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable or unstable luciferase/EGFP proteins. Stable and unstable types of luciferase/EGFP are suitable for analyzing the accumulation of and the real-time activity of NF-κB signal, respectively. Our findings suggest that the NAT system is effective for in vivo imaging of NF-κB signalling using cells or animals.
Relation: http://www.sciencedirect.com/science/journal/01652478
Type: article (author version)
URI: http://hdl.handle.net/2115/24260
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 畠山 鎮次

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