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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Establishment of a newly improved detection system for NF-κB activity
| Title: | Establishment of a newly improved detection system for NF-κB activity |
| Authors: | Matsuda, Mayuko Browse this author | | Tsukiyama, Tadasuke Browse this author | | Bohgaki, Miyuki Browse this author | | Nonomura, Katsuya Browse this author | | Hatakeyama, Shigetsugu Browse this author →KAKEN DB |
| Keywords: | NF-κB | | Transcription | | Reporter system | | Luciferase | | EGFP |
| Issue Date: | 15-Apr-2007 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Immunology Letters |
| Volume: | 109 |
| Issue: | 2 |
| Start Page: | 175 |
| End Page: | 181 |
| Publisher DOI: | 10.1016/j.imlet.2007.02.007 |
| PMID: | 17368808 |
| Abstract: | The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable or unstable luciferase/EGFP proteins. Stable and unstable types of luciferase/EGFP are suitable for analyzing the accumulation of and the real-time activity of NF-κB signal, respectively. Our findings suggest that the NAT system is effective for in vivo imaging of NF-κB signalling using cells or animals. |
| Relation: | http://www.sciencedirect.com/science/journal/01652478 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/24260 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 畠山 鎮次
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