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Establishment of a newly improved detection system for NF-κB activity
Title: | Establishment of a newly improved detection system for NF-κB activity |
Authors: | Matsuda, Mayuko Browse this author | Tsukiyama, Tadasuke Browse this author | Bohgaki, Miyuki Browse this author | Nonomura, Katsuya Browse this author | Hatakeyama, Shigetsugu Browse this author →KAKEN DB |
Keywords: | NF-κB | Transcription | Reporter system | Luciferase | EGFP |
Issue Date: | 15-Apr-2007 |
Publisher: | Elsevier B.V. |
Journal Title: | Immunology Letters |
Volume: | 109 |
Issue: | 2 |
Start Page: | 175 |
End Page: | 181 |
Publisher DOI: | 10.1016/j.imlet.2007.02.007 |
PMID: | 17368808 |
Abstract: | The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable or unstable luciferase/EGFP proteins. Stable and unstable types of luciferase/EGFP are suitable for analyzing the accumulation of and the real-time activity of NF-κB signal, respectively. Our findings suggest that the NAT system is effective for in vivo imaging of NF-κB signalling using cells or animals. |
Relation: | http://www.sciencedirect.com/science/journal/01652478 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/24260 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 畠山 鎮次
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