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Volume 41, Number 2-4 >

DEVELOPMENT OF HAPLOID AND DIPLOID MOUSE PARTHENOGENONES : EFFECT OF OOCYTE AGING IN VIVO

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.41.2-4.81

Title: DEVELOPMENT OF HAPLOID AND DIPLOID MOUSE PARTHENOGENONES : EFFECT OF OOCYTE AGING IN VIVO
Authors: PINYOPUMMIN, Anuchai Browse this author
TAKAHASHI, Yoshiyuki Browse this author
HISHINUMA, Mitsugu Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: Parthenogenesis
oocyte aging
development
mouse
Issue Date: 30-Nov-1993
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 41
Issue: 2-4
Start Page: 81
End Page: 87
Abstract: Mouse oocytes were collected 13.5-29.5 hr after hCG injection at 4 hr intervals, then activated with 7% ethanol for 1 min. The oocytes collected 13.5 hr after hCG injection did not respond to ethanol activation. High activation rates (90.2-98.3%) were obtained in oocytes activated 17.5-29.5 hr after injection with hCG. Haploid parthenogenones were dominant though the number decreased as the age of oocytes advanced (89.5% to 42.0%). The highest number of diploid parthenogenones (20.6%) was obtained in oocytes activated 21.5 hr after hCG injection. The number of immediate cleavage and morphologically abnormal oocytes increased when the oocyte age progressed (0.7% to 34.7% and 1.1% to 23.2%, respectively). The percentage of parthenogenones developing to blastocysts decreased with the increase in oocytes age : haploid 51.9% to 1.4% and diploid 100.0% to 83.3%. The present study demonstrates that the developmental potentials of haploid and diploid parthenogenones derived from oocytes at 17.5 and 21.5 hr are higher than those derived from oocytes at 25.5 and 29.5 hr after hCG injection.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2453
Appears in Collections:Japanese Journal of Veterinary Research > Volume 41, Number 2-4

Submitter: 獣医学部図書室

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