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Volume 42, Number 3-4 >

DEVELOPMENT OF SINGLE BLASTOMERES FROM 4-CELL STAGE EMBRYOS AFTER AGGREGATION WITH PARTHENOGENONES IN MICE

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.42.3-4.119

Title: DEVELOPMENT OF SINGLE BLASTOMERES FROM 4-CELL STAGE EMBRYOS AFTER AGGREGATION WITH PARTHENOGENONES IN MICE
Authors: PINYOPUMMIN, Anuchai Browse this author
TAKAHASHI, Yoshiyuki Browse this author
HISHINUMA, Mitsugu Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: Aggregation
4-cell embryo
parthenogenone
mouse
Issue Date: 31-Jan-1995
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 42
Issue: 3-4
Start Page: 119
End Page: 126
Abstract: This study was undertaken to determine whether diploid parthenogenones can assist the development of single blastomeres of 4-cell embryos to term. Isolated blastomeres of 4-cell embryos were aggregated with parthenogenones at the 2-, 4- and 8-cell stages. After the removal of the zonae, aggregation was done by pushing one blastomere of 4-cell embryos and one parthenogenone into contact using a micropipette. There were no significant differences in the percentages of blastocysts and offspring among all parthenogenones' developmental stages used. It also seemed that the chimerism of the offspring (judged by coat color) was not affected by the developmental stage of the parthenogenone. A total of 19 offspring survived and 12 offspring were coat-colored chimera. From 12 coat-colored chimeric offspring, 9 offspring had germlines derived from the isolated blastomeres of 4-cell embryos, one offspring had a germline derived from both the parthenogenone and the isolated blastomere of a 4-cell embryo and one offspring had a germline derived from the parthenogenone. One offspring however, was infertile. The present study demonstrates that parthenogenones can be used to assist the development of single blastomeres from 4-cell embryos to term.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2473
Appears in Collections:Japanese Journal of Veterinary Research > Volume 42, Number 3-4

Submitter: 獣医学部図書室

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