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Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.

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Title: Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.
Authors: Nakashima, A. Browse this author
Takaku, H. Browse this author
Shibata, H. Browse this author
Negishi, Y. Browse this author
Takagi, M. Browse this author
Tamura, M. Browse this author →KAKEN DB
Nashimoto, M. Browse this author
Keywords: gene silencing
tRNase Z
tRNase ZL
Issue Date: Jan-2007
Publisher: Nature Publishing Group
Journal Title: Gene Therapy
Volume: 14
Issue: 1
Start Page: 78
End Page: 85
Publisher DOI: 10.1038/
PMID: 16885998
Abstract: We have been developing a unique system for the downregulation of a gene expression through cutting a specific mRNA by the long form of tRNA 3'-processing endoribonuclease (tRNase ZL) under the direction of small-guide RNA (sgRNA). However, the efficacy of this system and the involvement of tRNase ZL in the living cells were not clear. Here we show, by targeting the exogenous luciferase gene, that the efficacy of the sgRNA/tRNase ZL method can become comparable to that of the RNA interference technology and that the gene silencing is owing to tRNase ZL directed by sgRNA not owing to a simple antisense effect. We also show that tRNase ZL together with sgRNA can downregulate expression of the endogenous human genes Bcl-2 and glycogen synthase kinase-3β by degrading their mRNAs in cell culture. Furthermore, we demonstrate that a gene expression in the livers of postnatal mice can be inhibited by an only seven-nucleotide sgRNA. These data suggest that sgRNA might be utilized as therapeutic agents to treat diseases such as cancers and AIDS.
Rights: Nature Publishing Group, Gene Therapy, Vol.14-1, 2007, pp. 78-85
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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