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Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.

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タイトル: Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.
著者: Nakashima, A. 著作を一覧する
Takaku, H. 著作を一覧する
Shibata, H. 著作を一覧する
Negishi, Y. 著作を一覧する
Takagi, M. 著作を一覧する
Tamura, M. 著作を一覧する
Nashimoto, M. 著作を一覧する
キーワード: gene silencing
RNAi
sgRNA
tRNase Z
tRNase ZL
発行日: 2007年 1月
出版者: Nature Publishing Group
誌名: Gene Therapy
巻: 14
号: 1
開始ページ: 78
終了ページ: 85
出版社 DOI: 10.1038/sj.gt.3302841
抄録: We have been developing a unique system for the downregulation of a gene expression through cutting a specific mRNA by the long form of tRNA 3'-processing endoribonuclease (tRNase ZL) under the direction of small-guide RNA (sgRNA). However, the efficacy of this system and the involvement of tRNase ZL in the living cells were not clear. Here we show, by targeting the exogenous luciferase gene, that the efficacy of the sgRNA/tRNase ZL method can become comparable to that of the RNA interference technology and that the gene silencing is owing to tRNase ZL directed by sgRNA not owing to a simple antisense effect. We also show that tRNase ZL together with sgRNA can downregulate expression of the endogenous human genes Bcl-2 and glycogen synthase kinase-3β by degrading their mRNAs in cell culture. Furthermore, we demonstrate that a gene expression in the livers of postnatal mice can be inhibited by an only seven-nucleotide sgRNA. These data suggest that sgRNA might be utilized as therapeutic agents to treat diseases such as cancers and AIDS.
Rights: Nature Publishing Group, Gene Therapy, Vol.14-1, 2007, pp. 78-85
関連URI: http://www.nature.com/npg/
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/25154
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田村 正人

 

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