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Volume 43, Number 3-4  >

EXPRESSION OF THE ENDOGENOUS MAREK'S DISEASE VIRUS ICP4 HOMOLOG (MDV ICP4) GENE IS ENHANCED IN LATENTLY INFECTED CELLS BY TRANSIENT TRANSFECTION WITH THE RECOMBINANT MDV ICP4 GENE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.43.3-4.109

Title: EXPRESSION OF THE ENDOGENOUS MAREK'S DISEASE VIRUS ICP4 HOMOLOG (MDV ICP4) GENE IS ENHANCED IN LATENTLY INFECTED CELLS BY TRANSIENT TRANSFECTION WITH THE RECOMBINANT MDV ICP4 GENE
Authors: ENDOH, Daiji Browse this author
IKEGAWA, Satoshi Browse this author
KON, Yasuhiro Browse this author →KAKEN DB
HAYASHI, Masanobu Browse this author
SATO, Fumiaki Browse this author
Keywords: Marek's disease virus
ICP4 homolog
High mobility group proteins
Cationic liposomes
Virus replication
Issue Date: 27-Dec-1995
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 43
Issue: 3-4
Start Page: 109
End Page: 124
Abstract: The ICP4 homolog of Marek's disease virus (MDV ICP4) is a possible candidate for the transactivator of the early genes. We transfected MDCC- MSB-1 (MSB-1) tumor cells with plasmid including a coding region of MDV ICP4 using cationic liposome. As carriers for intranuclear transport, high mobility group -1 and -2 proteins were bound to the plasmid DNA before forming liposomes. We detected transcripts from the plasmid 2 hr after transfection by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis. We also detected abundant transcripts of endogenous ICP4 2-96 hr after transfection. These data suggested that expression of introduced MDV ICP4 gene enhanced the expression of endogenous MDV ICP4. On the other hand, quantitative PCR analysis for virus genome DNA indicated no significant alteration of copy number of virus genome in transfected MSB-1 cells, suggesting that reactivation of virus requires more than turning on MDV ICP4 gene.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2516
Appears in Collections:Japanese Journal of Veterinary Research > Volume 43, Number 3-4

Submitter: 獣医学部図書室

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