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Volume 43, Number 3-4  >

ELECTROFUSION OF ZONA-FREE MOUSE EMBRYONIC CELLS IN ELECTROLYTES AND THEIR DEVELOPMENT IN VITRO

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.43.3-4.125

Title: ELECTROFUSION OF ZONA-FREE MOUSE EMBRYONIC CELLS IN ELECTROLYTES AND THEIR DEVELOPMENT IN VITRO
Authors: ELSHEIKH, Adil S. Browse this author
TAKAHASHI, Yoshiyuki Browse this author
TANAKA, Hozumi Browse this author
HISHINUMA, Mitsugu Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: Electrofusion
electrolytes
mouse strain
zona-free embryonic cells
Issue Date: 27-Dec-1995
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 43
Issue: 3-4
Start Page: 125
End Page: 134
Abstract: The influence of increasing the physical electrofusion parameters, direct current (DC) pulse strength, pulse duration, pulse number, alternating current (AC) voltage and alignment time, in electrolytes on the rates of fusion, degeneration and development of zona-free mouse 2-cell embryos were examined. Furthermore, the effects of physiological saline and mannitol as fusion media and various mouse strains were also evaluated. Dulbecco's phosphate-buffered saline (PBS) supplemented with 10% fetal calf serum was used as the main fusion solution. A significant increase in the rate of fusion (P<0.05) was obtained by increasing pulse strength from 30 to 300 V/mm. The embryos fused at the pulse strengths of 30 to 70 V/mm had significantly higher development rates to blastocysts compared with those fused at 100 to 300 V/mm (P<0.05). There were no significant differences in the rates of fusion, degeneration and development to blastocysts when the pulse duration was increased from 30 to 90 μsec. Although fusion rates were increased (P<0.05) by increasing the pulse number up to 4,a significant decrease (P<0.05) in development to blastocysts was observed when the pulse number was 5. Application of AC voltage prior to the DC pulse tended to increase the fusion rate (89.2-93.8%), compared with fusion with the DC pulse only (75.0%). Prolongation of alignment time from 5 to 15 sec had no effect on the fusion rate. Under the optimum conditions (2 pulses of DC of 70 V/mm, 70 μsec pulse duration and AC of 5 V/mm for 5 sec), no significant difference was obtained in the fusion and development rates in different mouse strains, nor were fusion and development rates significantly different among PBS, physiological saline and mannitol solutions (P>0.05).
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2517
Appears in Collections:Japanese Journal of Veterinary Research > Volume 43, Number 3-4

Submitter: 獣医学部図書室

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