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Volume 45, Number 4 >

Current status of vitrification of embryos and oocytes in domestic animals : Ethylene glycol as an emerging cryoprotectant of choice

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.45.4.183

タイトル: Current status of vitrification of embryos and oocytes in domestic animals : Ethylene glycol as an emerging cryoprotectant of choice
著者: BAUTISTA, Jose Arceo N. 著作を一覧する
KANAGAWA, Hiroshi 著作を一覧する
キーワード: cryoprotectant
embryos
ethylene glycol
oocytes
vitrification
発行日: 1998年 2月27日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 45
号: 4
開始ページ: 183
終了ページ: 191
抄録: The cryopreservation of mammalian embryos has become an integral part of methods to control animal reproduction. Numerous vitrification solutions have been formulated with ethylene glycol in combination with macromolecules, sugars and other cryoprotective agents. These indicate that a study of ethylene glycol as a cryoprotectant of choice in vitrification studies would be promising. To understand the cryobiology of ethylene glycol, several factors have to be studied. These are : cryoprotectant toxicity, osmotic stress and temperature at exposure. Understanding these factors could lead to the formulation of vitrification protocols that would lead to higher viability rates after cooling. First, ethylene glycol must be used as the sole cryoprotectant in a solution without macromolecules and sugars. Second, partial dehydration and permeation prior to cooling to subzero temperatures must be studied to achieve accurate exposure and a one-step dilution method. Third, the toxic effects of ethylene glycol must be overcome without sacrificing its vitrification properties by combining step-wise exposure at appropriate temperatures, low concentration and decreased volume. Fourth, the long-term effects of ethylene glycol on exposed or vitrified embryos must be determined. Lastly, the influence of culture on the viability of vitrified embryos must be studied to improve viability rates after warming.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2610
出現コレクション:Volume 45, Number 4

提供者: 獣医学部図書室

 

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