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Volume 45, Number 4 >

Current status of vitrification of embryos and oocytes in domestic animals : Ethylene glycol as an emerging cryoprotectant of choice

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.45.4.183

Title: Current status of vitrification of embryos and oocytes in domestic animals : Ethylene glycol as an emerging cryoprotectant of choice
Authors: BAUTISTA, Jose Arceo N. Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: cryoprotectant
embryos
ethylene glycol
oocytes
vitrification
Issue Date: 27-Feb-1998
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 45
Issue: 4
Start Page: 183
End Page: 191
Abstract: The cryopreservation of mammalian embryos has become an integral part of methods to control animal reproduction. Numerous vitrification solutions have been formulated with ethylene glycol in combination with macromolecules, sugars and other cryoprotective agents. These indicate that a study of ethylene glycol as a cryoprotectant of choice in vitrification studies would be promising. To understand the cryobiology of ethylene glycol, several factors have to be studied. These are : cryoprotectant toxicity, osmotic stress and temperature at exposure. Understanding these factors could lead to the formulation of vitrification protocols that would lead to higher viability rates after cooling. First, ethylene glycol must be used as the sole cryoprotectant in a solution without macromolecules and sugars. Second, partial dehydration and permeation prior to cooling to subzero temperatures must be studied to achieve accurate exposure and a one-step dilution method. Third, the toxic effects of ethylene glycol must be overcome without sacrificing its vitrification properties by combining step-wise exposure at appropriate temperatures, low concentration and decreased volume. Fourth, the long-term effects of ethylene glycol on exposed or vitrified embryos must be determined. Lastly, the influence of culture on the viability of vitrified embryos must be studied to improve viability rates after warming.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2610
Appears in Collections:Japanese Journal of Veterinary Research > Volume 45, Number 4

Submitter: 獣医学部図書室

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