HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 45, Number 4 >

In vitro viability of mouse zygotes vitrified in ethylene glycol

Files in This Item:
KJ00002398577.pdf577.04 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.45.4.193

Title: In vitro viability of mouse zygotes vitrified in ethylene glycol
Authors: BAUTISTA, Jose Arceo N. Browse this author
TAKAHASHI, Yoshiyuki Browse this author →KAKEN DB
KANAGAWA, Hiroshi Browse this author
Keywords: culture
embryos
ethylene glycol
mouse
vitrification
Issue Date: 27-Feb-1998
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 45
Issue: 4
Start Page: 193
End Page: 198
Abstract: A study was made to determine if mouse zygotes can be effectively vitrified in 7 M ethylene glycol in modified Dulbecco's phosphate buffered saline (PB1) and to find out if the development of vitrified-warmed zygotes in vitro can be improved by renewing the culture medium. The results showed that without medium change, vitrification reduced the development of zygotes to the expanded blastocyst stage (p<0.01). With medium change, the development rate of vitrified-warmed zygotes exposed in 7 M ethylene glycol for 1 or 2 min was similar to that of unvitrified zygotes. However, prolonged exposure (5 min) markedly reduced the development rates of vitrified-warmed zygotes to the expanded blastocyst stage (p<0.05). When the zygotes were vitrified in 7 M ethylene glycol and diluted at 18℃ to 22℃, a slower efflux of ethylene glycol from the cell might have occurred, leading to a toxic effect of ethylene glycol in culture. The development rates of vitrified embryos cultured with medium change at 24 hr did not significantly differ from the untreated control (89.0% vs 96.5%). In conclusion, this study showed that mouse zygotes can be vitrified in 7 M ethylene glycol in PB1 and that changing the culture medium can improve the in vitro development rates of vitrified-warmed zygotes to the expanded blastocyst stage.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2611
Appears in Collections:Japanese Journal of Veterinary Research > Volume 45, Number 4

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University