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Volume 45, Number 4 >

Functional enucleation of mouse metaphase II oocytes with etoposide

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.45.4.217

Title: Functional enucleation of mouse metaphase II oocytes with etoposide
Authors: ELSHEIKH, Adil Salim Browse this author
TAKAHASHI, Yoshiyuki Browse this author →KAKEN DB
KATAGIRI, Seiji Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: Enucleation
etoposide
reconstituted embryos
Issue Date: 27-Feb-1998
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 45
Issue: 4
Start Page: 217
End Page: 220
Abstract: Mouse metaphase II (M II) oocytes were exposed to 50 μg/ml etoposide (ETO) before and after parthenogenetic activation with 7% ethanol and they were washed with 0.75 M sucrose. The ETO treated parthenogenetically activated oocytes were cultured or fused to single blastomeres of late 2-cell stage mouse embryo to test their ability to support development in vitro. In parallel untreated parthenogenetically activated oocytes were cultured to serve as control. None of ETO treated oocytes developed beyond the 2-cell stage, whereas 4% of the reconstituted embryos and 35% of control developed to blastocysts. It is concluded that mouse M II oocytes can be functionally enucleated by ETO treatment and can be used for nuclear transfer experiments.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2614
Appears in Collections:Japanese Journal of Veterinary Research > Volume 45, Number 4

Submitter: 獣医学部図書室

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