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Volume 45, Number 4 >

Functional enucleation of mouse metaphase II oocytes with etoposide

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タイトル: Functional enucleation of mouse metaphase II oocytes with etoposide
著者: ELSHEIKH, Adil Salim 著作を一覧する
TAKAHASHI, Yoshiyuki 著作を一覧する
KATAGIRI, Seiji 著作を一覧する
KANAGAWA, Hiroshi 著作を一覧する
キーワード: Enucleation
reconstituted embryos
発行日: 1998年 2月27日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 45
号: 4
開始ページ: 217
終了ページ: 220
抄録: Mouse metaphase II (M II) oocytes were exposed to 50 μg/ml etoposide (ETO) before and after parthenogenetic activation with 7% ethanol and they were washed with 0.75 M sucrose. The ETO treated parthenogenetically activated oocytes were cultured or fused to single blastomeres of late 2-cell stage mouse embryo to test their ability to support development in vitro. In parallel untreated parthenogenetically activated oocytes were cultured to serve as control. None of ETO treated oocytes developed beyond the 2-cell stage, whereas 4% of the reconstituted embryos and 35% of control developed to blastocysts. It is concluded that mouse M II oocytes can be functionally enucleated by ETO treatment and can be used for nuclear transfer experiments.
資料タイプ: bulletin
出現コレクション:Volume 45, Number 4


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