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Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.
|Title: ||Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.|
|Authors: ||Yasui, Hironobu Browse this author →KAKEN DB|
|Inanami, Osamu Browse this author →KAKEN DB|
|Asanuma, Taketoshi Browse this author|
|Iizuka, Daisuke Browse this author|
|Nakajima, Takayuki Browse this author|
|Kon, Yasuhiro Browse this author →KAKEN DB|
|Matsuda, Akira Browse this author →KAKEN DB|
|Kuwabara, Mikinori Browse this author|
|Keywords: ||Transplanted tumor|
|Issue Date: ||1-May-2007|
|Publisher: ||Elsevier Inc.|
|Journal Title: ||International Journal of Radiation OncologyBiologyPhysics|
|Start Page: ||218|
|End Page: ||228|
|Publisher DOI: ||10.1016/j.ijrobp.2006.12.061|
|Abstract: ||Purpose: To examine the in vivo antitumor efficacy of X-irradiation combined with administration of a ribonucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106, ECyd), to tumor cell–transplanted mice.
Methods and Materials: Colon26 murine rectum adenocarcinoma cells and MKN45 human gastric adenocarcinoma cells were inoculated into the footpad in BALB/c mice and severe combined immunodeficient mice, respectively. They were treated with a relatively low dose of X-irradiation (2 Gy) and low amounts of TAS106 (0.1 mg/kg and 0.5 mg/kg). The tumor growth was monitored by measuring the tumor volume from Day 5 to Day 16 for Colon26 and from Day 7 to Day 20 for MKN45. Histologic analyses for proliferative and apoptotic cells in the tumors were performed using Ki-67 immunohistochemical and terminal deoxynucleotidyl transferase-mediated nick end labeling staining. The expression of survivin, a key molecule related to tumor survival, was assessed by quantitative polymerase chain reaction and immunohistochemical analysis.
Results: When X-irradiation and TAS106 treatment were combined, significant inhibition of tumor growth was observed in both types of tumors compared with mice treated with X-irradiation or TAS106 alone. Marked inhibition of tumor growth was observed in half of the mice that received the combined treatment three times at 2-day intervals. Parallel to these phenomena, the suppression of survivin expression and appearance of Ki-67–negative and apoptotic cells were observed.
Conclusions: X-irradiation and TAS106 effectively suppress tumor growth in mice. The inhibition of survivin expression by TAS106 is thought to mainly contribute to the suppression of the tumor growth.|
|Type: ||article (author version)|
|Appears in Collections:||獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)|
Submitter: 稲波 修