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Hokkaido University Collection of Scholarly and Academic Papers >
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Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice.
| Title: | Treatment combining X-irradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice. |
| Authors: | Yasui, Hironobu Browse this author →KAKEN DB | | Inanami, Osamu Browse this author →KAKEN DB | | Asanuma, Taketoshi Browse this author | | Iizuka, Daisuke Browse this author | | Nakajima, Takayuki Browse this author | | Kon, Yasuhiro Browse this author →KAKEN DB | | Matsuda, Akira Browse this author →KAKEN DB | | Kuwabara, Mikinori Browse this author |
| Keywords: | Transplanted tumor | | Anticancer drug | | X-irradiation | | Survivin |
| Issue Date: | 1-May-2007 |
| Publisher: | Elsevier Inc. |
| Journal Title: | International Journal of Radiation OncologyBiologyPhysics |
| Volume: | 68 |
| Issue: | 1 |
| Start Page: | 218 |
| End Page: | 228 |
| Publisher DOI: | 10.1016/j.ijrobp.2006.12.061 |
| PMID: | 17448876 |
| Abstract: | Purpose: To examine the in vivo antitumor efficacy of X-irradiation combined with administration of a ribonucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106, ECyd), to tumor cell–transplanted mice.
Methods and Materials: Colon26 murine rectum adenocarcinoma cells and MKN45 human gastric adenocarcinoma cells were inoculated into the footpad in BALB/c mice and severe combined immunodeficient mice, respectively. They were treated with a relatively low dose of X-irradiation (2 Gy) and low amounts of TAS106 (0.1 mg/kg and 0.5 mg/kg). The tumor growth was monitored by measuring the tumor volume from Day 5 to Day 16 for Colon26 and from Day 7 to Day 20 for MKN45. Histologic analyses for proliferative and apoptotic cells in the tumors were performed using Ki-67 immunohistochemical and terminal deoxynucleotidyl transferase-mediated nick end labeling staining. The expression of survivin, a key molecule related to tumor survival, was assessed by quantitative polymerase chain reaction and immunohistochemical analysis.
Results: When X-irradiation and TAS106 treatment were combined, significant inhibition of tumor growth was observed in both types of tumors compared with mice treated with X-irradiation or TAS106 alone. Marked inhibition of tumor growth was observed in half of the mice that received the combined treatment three times at 2-day intervals. Parallel to these phenomena, the suppression of survivin expression and appearance of Ki-67–negative and apoptotic cells were observed.
Conclusions: X-irradiation and TAS106 effectively suppress tumor growth in mice. The inhibition of survivin expression by TAS106 is thought to mainly contribute to the suppression of the tumor growth. |
| Relation: | http://www.sciencedirect.com/science/journal/03603016 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/26187 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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