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Volume 46, Number 4 >

Characterization of proteolytic enzymes expressed in the midgut of Haemaphysalis longicornis

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Title: Characterization of proteolytic enzymes expressed in the midgut of Haemaphysalis longicornis
Authors: MULENGA, Albert Browse this author
SUGIMOTO, Chihiro Browse this author
ONUMA, Misao Browse this author
Keywords: Cysteine proteinases
Haemaphysalis longicornis
midgut proteolytic enzymes
Issue Date: 26-Feb-1999
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 46
Issue: 4
Start Page: 179
End Page: 184
Abstract: The proteolytic activities present in midguts of both fed and unfed Haemaphysalis longicornis were assessed by using the gelatin-substrate gel electrophoresis and inhibitor sensitivity analyses. Three predominant (116,48 and 40 kDa) and two weak (55 and 60 kDa) proteinase bands were commonly expressed in both unfed and fed ticks, while a weak 80 kDa band was only present in fed ticks. Consistent with observations on other tick species, proteolytic activity against the gelatin substrate was observed only under acidic conditions. Inhibition studies against the gelatin substrate using a panel of inhibitors showed that the predominant proteolytic enzymes of 40 and 48 kDa molecular mass are cysteine proteinases. These results are discussed in the context of host vaccination as an alternative tick control method to the current use of chemical acaricides.
Type: bulletin (article)
Appears in Collections:Japanese Journal of Veterinary Research > Volume 46, Number 4

Submitter: 獣医学部図書室

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