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Volume 47, Number 3-4 >

Application of FT-IR and ESR spectroscopic techniques to the study of CCl_4-induced peroxidation in rat liver microsomes

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.47.3-4.151

タイトル: Application of FT-IR and ESR spectroscopic techniques to the study of CCl_4-induced peroxidation in rat liver microsomes
著者: YOON, Seokjoo 著作を一覧する
MARUYAMA, Yutaka 著作を一覧する
KAZUSAKA, Akio 著作を一覧する
FUJITA, Shoichi 著作を一覧する
キーワード: lipid peroxidation
liver microsomes
spectroscopy
発行日: 2000年 2月29日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 47
号: 3-4
開始ページ: 151
終了ページ: 154
抄録: FT-IR and ESR were used for on the investigation of the CCl_4-induced peroxidation of rat liver microsomes in combination with biochemical methods. Lipid peroxidation was assayed by TBA reagent in the presence of CCl_4 and NADPH. The CCl_3 radical was detected by ESR spectroscopy with a spin trapping reagent of PBN. The FT-IR spectroscopy revealed that absorption band of -C-H in -C=C-H decreased in intensity at 3012 cm^<-1>, but the absorption bands of the phosphate head and choline in the phospholipids did not significantly change between 1300 and 900 cm^<-1>. These findings were interpreted to be due to the removal of H from -C=C-H by radicals as the first step of lipid peroxidation, and to the absence of dephosphorylation of phospholipids in the microsomal membrane. This is the first IR spectroscopic evidence indicating the nature of damage to a microsomal membrane caused by CCl_4 treatment. The spectroscopies used here demonstrated that they are useful tools to observe the damage to microsomal membranes.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2789
出現コレクション:Volume 47, Number 3-4

提供者: 獣医学部図書室

 

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