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Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus

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Title: Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus
Authors: Uraki, Yasumitsu Browse this author →KAKEN DB
Nemoto, Junji Browse this author
Otsuka, Hiroyuki Browse this author
Tamai, Yutaka Browse this author →KAKEN DB
Sugiyama, Junji Browse this author
Kishimoto, Takao Browse this author
Ubukata, Makoto Browse this author →KAKEN DB
Yabu, Hiroshi Browse this author
Tanaka, Masaru Browse this author
Shimomura, Masatsugu Browse this author
Keywords: Cellulose Iα
Electron diffraction
Honeycomb-patterned bacterial cellulose
Self-organization
Issue Date: 1-May-2007
Publisher: Elsevier
Journal Title: Carbohydrate Polymers
Volume: 69
Issue: 1
Start Page: 1
End Page: 6
Publisher DOI: 10.1016/j.carbpol.2006.08.021
Abstract: Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO2 atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose Iα microfibrils. As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC.
Relation: http://www.sciencedirect.com/science/journal/01448617
Type: article (author version)
URI: http://hdl.handle.net/2115/27989
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 浦木 康光

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