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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows.
| Title: | Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows. |
| Authors: | AHMED, Mohamed Browse this author | | SHABAN, Zein Browse this author | | YAMAJI, Daisuke Browse this author | | OKAMATSU-OGURA, Yuko Browse this author | | SOLIMAN, Mohamed Browse this author | | ABD ELDAIM, Mabrouk Browse this author | | ISHIOKA, Katsumi Browse this author | | MAKONDO, Kennedy Browse this author | | SAITO, Masayuki Browse this author | | KIMURA, Kazuhiro Browse this author →KAKEN DB |
| Keywords: | caspase-1 | | IL-1 | | IL-12 | | IL-18 | | leptin |
| Issue Date: | May-2007 |
| Publisher: | 社団法人 日本獣医学会 |
| Journal Title: | Journal of Veterinary Medical Science |
| Volume: | 69 |
| Issue: | 5 |
| Start Page: | 509 |
| End Page: | 514 |
| Publisher DOI: | 10.1292/jvms.69.509 |
| PMID: | 17551224 |
| Abstract: | The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated with either leptin alone or combination of leptin and ConA showed enhanced proliferative activity by 10-40%, compared with those treated with ConA alone. In contrast, isolated T lymphocytes treated with leptin and ConA showed lowered proliferative activity than the ConA-treated alone, indicating that leptin induced production of some cytokines from monocyte/macrophages, that subsequently resulted in enhancement of T lymphocytes proliferation in PBMC. Among the cytokines examined, monocyte/monocytes constitutively expressed interleukin (IL)-1β, IL-12p35, IL-18 mRNA, and faintly expressed tumor necrosis factor (TNF)-α and IL-12p40 mRNA. Leptin treatment augmented the monocyte/macrophages mRNA expression of only TNF-α and IL-12p40 to comparable levels of cells treated with lipopolysaccharide (LPS). However, leptin treatment increased monocyte/macrophages production of IL-1β as well as TNF-α, and induced the mRNA expression of caspase-1, which is shown to mediate the conversion of latent pro-IL-1β and pro-IL-18 to active forms. These results suggest that leptin directly acts on monocyte/macrophages to produce factors that induce T lymphocytes proliferation such as IL-12p35/p40 complex through IL-12p40 induction and IL-1β/IL-18 production through caspase-1 induction. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/27992 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 和弘
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