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Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows.

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Title: Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows.
Authors: AHMED, Mohamed Browse this author
SHABAN, Zein Browse this author
YAMAJI, Daisuke Browse this author
OKAMATSU-OGURA, Yuko Browse this author
SOLIMAN, Mohamed Browse this author
ABD ELDAIM, Mabrouk Browse this author
ISHIOKA, Katsumi Browse this author
MAKONDO, Kennedy Browse this author
SAITO, Masayuki Browse this author
KIMURA, Kazuhiro Browse this author →KAKEN DB
Keywords: caspase-1
Issue Date: May-2007
Publisher: 社団法人 日本獣医学会
Journal Title: Journal of Veterinary Medical Science
Volume: 69
Issue: 5
Start Page: 509
End Page: 514
Publisher DOI: 10.1292/jvms.69.509
PMID: 17551224
Abstract: The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated with either leptin alone or combination of leptin and ConA showed enhanced proliferative activity by 10-40%, compared with those treated with ConA alone. In contrast, isolated T lymphocytes treated with leptin and ConA showed lowered proliferative activity than the ConA-treated alone, indicating that leptin induced production of some cytokines from monocyte/macrophages, that subsequently resulted in enhancement of T lymphocytes proliferation in PBMC. Among the cytokines examined, monocyte/monocytes constitutively expressed interleukin (IL)-1β, IL-12p35, IL-18 mRNA, and faintly expressed tumor necrosis factor (TNF)-α and IL-12p40 mRNA. Leptin treatment augmented the monocyte/macrophages mRNA expression of only TNF-α and IL-12p40 to comparable levels of cells treated with lipopolysaccharide (LPS). However, leptin treatment increased monocyte/macrophages production of IL-1β as well as TNF-α, and induced the mRNA expression of caspase-1, which is shown to mediate the conversion of latent pro-IL-1β and pro-IL-18 to active forms. These results suggest that leptin directly acts on monocyte/macrophages to produce factors that induce T lymphocytes proliferation such as IL-12p35/p40 complex through IL-12p40 induction and IL-1β/IL-18 production through caspase-1 induction.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 和弘

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