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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows.
| Title: | Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows. |
| Authors: | AHMED, Mohamed Browse this author | | KIMURA, Kazuhiro Browse this author →KAKEN DB | | SOLIMAN, Mohamed Browse this author | | YAMAJI, Daisuke Browse this author | | OKAMATSU-OGURA, Yuko Browse this author | | MAKONDO, Kennedy Browse this author | | INANAMI, Osamu Browse this author →KAKEN DB | | SAITO, Masayuki Browse this author |
| Keywords: | degranulation | | NADPH oxidase | | Ob-Rb | | p47phox | | TNF-α |
| Issue Date: | Feb-2007 |
| Publisher: | 社団法人 日本獣医学会 |
| Journal Title: | Journal of Veterinary Medical Science |
| Volume: | 69 |
| Issue: | 2 |
| Start Page: | 125 |
| End Page: | 131 |
| Publisher DOI: | 10.1292/jvms.69.125 |
| PMID: | 17339755 |
| Abstract: | Leptin, a pleiotropic hormone regulating food intake and energy expenditure, has been shown to directly modulate human polymorphonuclear neutrophil (PMN) functions or indirectly through the action of tumor necrosis factor-α (TNF-α). Bovine PMN have considerable different characteristics from human PMN. For example, it does not respond to N-formyl-Methionyl-Leucyl-phenylalanine, a well known human PMN activator. In the present study, we tested the effects of leptin and TNF-α on superoxide production and degranulation of bovine peripheral PMN, in which both long isoform of leptin receptor (Ob-Rb) and TNF receptor 1 were expressed. Human leptin, human TNF-α, phorbol myristate acetate (PMA) and opsonized zymosan particles (OZP) did not stimulate degranulation responses, while zymosan-activated serum (ZAS) did. Neither leptin nor TNF-α enhanced the ZAS-induced degranulation responses. TNF-α, PMA, OZP and ZAS increased superoxide production in different magnitudes, whereas leptin did not. TNF-α, but not leptin, enhanced OZP- and ZAS-induced superoxide production, possibly, in part due to facilitating translocation of p47phox, a component of NADPH oxidase. These results indicate that, unlike in human PMN, leptin does not have any direct effect on degranulation and superoxide production in bovine PMN, although TNF-α influences superoxide production. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/27993 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 和弘
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