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Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows.

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Title: Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows.
Authors: AHMED, Mohamed Browse this author
KIMURA, Kazuhiro Browse this author →KAKEN DB
SOLIMAN, Mohamed Browse this author
YAMAJI, Daisuke Browse this author
OKAMATSU-OGURA, Yuko Browse this author
MAKONDO, Kennedy Browse this author
INANAMI, Osamu Browse this author →KAKEN DB
SAITO, Masayuki Browse this author
Keywords: degranulation
NADPH oxidase
Ob-Rb
p47phox
TNF-α
Issue Date: Feb-2007
Publisher: 社団法人 日本獣医学会
Journal Title: Journal of Veterinary Medical Science
Volume: 69
Issue: 2
Start Page: 125
End Page: 131
Publisher DOI: 10.1292/jvms.69.125
PMID: 17339755
Abstract: Leptin, a pleiotropic hormone regulating food intake and energy expenditure, has been shown to directly modulate human polymorphonuclear neutrophil (PMN) functions or indirectly through the action of tumor necrosis factor-α (TNF-α). Bovine PMN have considerable different characteristics from human PMN. For example, it does not respond to N-formyl-Methionyl-Leucyl-phenylalanine, a well known human PMN activator. In the present study, we tested the effects of leptin and TNF-α on superoxide production and degranulation of bovine peripheral PMN, in which both long isoform of leptin receptor (Ob-Rb) and TNF receptor 1 were expressed. Human leptin, human TNF-α, phorbol myristate acetate (PMA) and opsonized zymosan particles (OZP) did not stimulate degranulation responses, while zymosan-activated serum (ZAS) did. Neither leptin nor TNF-α enhanced the ZAS-induced degranulation responses. TNF-α, PMA, OZP and ZAS increased superoxide production in different magnitudes, whereas leptin did not. TNF-α, but not leptin, enhanced OZP- and ZAS-induced superoxide production, possibly, in part due to facilitating translocation of p47phox, a component of NADPH oxidase. These results indicate that, unlike in human PMN, leptin does not have any direct effect on degranulation and superoxide production in bovine PMN, although TNF-α influences superoxide production.
Type: article (author version)
URI: http://hdl.handle.net/2115/27993
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 和弘

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