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Immunogenicity and Protective Efficacy of Replication-Incompetent Influenza Virus-Like Particles

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Title: Immunogenicity and Protective Efficacy of Replication-Incompetent Influenza Virus-Like Particles
Authors: Watanabe, Tokiko Browse this author
Watanabe, Shinji Browse this author
Neumann, Gabriele Browse this author
Kida, Hiroshi Browse this author →KAKEN DB
Kawaoka, Yoshihiro Browse this author →KAKEN DB
Issue Date: Jan-2002
Publisher: American Society for Microbiology
Journal Title: Journal of Virology
Volume: 76
Issue: 2
Start Page: 767
End Page: 773
Publisher DOI: 10.1128/JVI.76.2.767-773.2002
PMID: 11752166
Abstract: Despite the success of influenza virus vaccines in reducing severe illness, their efficacy is suboptimal. We describe here the immunogenicity and protective capacity of replication-incompetent influenza virus-like particles (VLPs) which were generated entirely from cDNAs and lacked either the entire NS gene (encoding both the NS1 and NS2 protein) or only the NS2 gene. In mammalian cells infected with NS gene-deficient VLPs, the nucleoprotein, but not other viral proteins including hemagglutinin (HA) and neuraminidase (NA), was detected. In contrast, cells infected with VLPs expressing NS1 but not NS2 (NS2 knockout) expressed multiple viral proteins, including HA and NA. When challenged with lethal doses of an antigenically homologous mouse-adapted influenza virus, 94% of mice vaccinated with the NS2-knockout VLPs survived, compared with less than 10% of those given the NS-deficient VLPs. These results demonstrate the potential of replication-incompetent NS2-knockout VLPs as novel influenza vaccines and perhaps also as vectors to express genes from entirely unrelated pathogens.
Rights: Copyright ©2002 American Society for Microbiology
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 喜田 宏

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