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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Influenza A Virus Can Undergo Multiple Cycles of Replication without M2 Ion Channel Activity
| Title: | Influenza A Virus Can Undergo Multiple Cycles of Replication without M2 Ion Channel Activity |
| Authors: | Watanabe, Tokiko Browse this author | | Watanabe, Shinji Browse this author | | Ito, Hiroshi Browse this author | | Kida, Hiroshi Browse this author →KAKEN DB | | Kawaoka, Yoshihiro Browse this author →KAKEN DB |
| Issue Date: | Jun-2001 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of Virology |
| Volume: | 75 |
| Issue: | 12 |
| Start Page: | 5656 |
| End Page: | 5662 |
| Publisher DOI: | 10.1128/JVI.75.12.5656-5662.2001 |
| PMID: | 11356973 |
| Abstract: | Ion channel proteins are common constituents of cells and have even been identified in some viruses. For example, the M2 protein of influenza A virus has proton ion channel activity that is thought to play an important role in viral replication. Because direct support for this function is lacking, we attempted to generate viruses with defective M2 ion channel activity. Unexpectedly, mutants with apparent loss of M2 ion channel activity by an in vitro assay replicated as efficiently as the wild-type virus in cell culture. We also generated a chimeric mutant containing an M2 protein whose transmembrane domain was replaced with that from the hemagglutinin glycoprotein. This virus replicated reasonably well in cell culture but showed no growth in mice. Finally, a mutant lacking both the transmembrane and cytoplasmic domains of M2 protein grew poorly in cell culture and showed no growth in mice. Thus, influenza A virus can undergo multiple cycles of replication without the M2 transmembrane domain responsible for ion channel activity, although this activity promotes efficient viral replication. |
| Rights: | Copyright © 2001 American Society for Microbiology |
| Type: | article |
| URI: | http://hdl.handle.net/2115/28144 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 喜田 宏
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