| Title: | Recognition of N-Glycolylneuraminic Acid Linked to Galactose by the α2,3 Linkage Is Associated with Intestinal Replication of Influenza A Virus in Ducks |
| Authors: | Ito, Toshihiro Browse this author |
| Suzuki, Yasuo Browse this author |
| Suzuki, Takashi Browse this author →KAKEN DB |
| Takada, Ayato Browse this author →KAKEN DB |
| Horimoto, Taisuke Browse this author →KAKEN DB |
| Wells, Krisna Browse this author |
| Kida, Hiroshi Browse this author →KAKEN DB |
| Otsuki, Koichi Browse this author |
| Kiso, Makoto Browse this author →KAKEN DB |
| Ishida, Hideharu Browse this author →KAKEN DB |
| Kawaoka, Yoshihiro Browse this author →KAKEN DB |
| Issue Date: | Oct-2000 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Journal of Virology |
| Volume: | 74 |
| Issue: | 19 |
| Start Page: | 9300 |
| End Page: | 9305 |
| Publisher DOI: | 10.1128/JVI.74.19.9300-9305.2000 |
| PMID: | 10982377 |
| Abstract: | The hemagglutinin (HA) of H3 human influenza viruses does not support viral replication in duck intestine despite its avian origin. A Leu-to-Gln mutation at position 226 and a Ser-to-Gly mutation at position 228 in the HA of human A/Udorn/307/72 (H3N2) permit a reassortant virus [human Udorn HA, with all other genes from A/mallard/New York/6750/78 (H2N2)] to replicate in ducks. To understand the molecular basis of this change in host range restriction, we investigated the receptor specificity of duck influenza viruses as well as of human-duck virus reassortants. The results indicate that the recognition of a glycoconjugate moiety possessing N-glycolneuramic acid (NeuGc) linked to galactose by the α2,3 linkage (NeuGcα2,3Gal) is associated with viral replication in duck intestine. Immunofluorescence assays with NeuGcα2,3Gal-specific antiserum detected this moiety primarily on the crypt epithelial cells of duck colon. Such recognition, together with biochemical evidence of NeuGc in crypt cells, correlated exactly with the ability of the virus to replicate in duck colon. These results suggest that recognition of the NeuGcα2,3-Gal moiety plays an important role in the enterotropism of avian influenza viruses. |
| Rights: | Copyright © 2000 American Society for Microbiology |
| Type: | article |
| URI: | http://hdl.handle.net/2115/28146 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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