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Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions

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Title: Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions
Authors: Muramoto, Yukiko Browse this author
Takada, Ayato Browse this author →KAKEN DB
Fujii, Ken Browse this author
Noda, Takeshi Browse this author
Iwatsuki-Horimoto, Kiyoko Browse this author
Watanabe, Shinji Browse this author
Horimoto, Taisuke Browse this author →KAKEN DB
Kida, Hiroshi8 Browse this author →KAKEN DB
Kawaoka, Yoshihiro Browse this author →KAKEN DB
Authors(alt): 喜田, 宏8
Issue Date: Mar-2006
Publisher: American Society for Microbiology
Journal Title: Journal of Virology
Volume: 80
Issue: 5
Start Page: 2318
End Page: 2325
Publisher DOI: 10.1128/JVI.80.5.2318-2325.2006
PMID: 16474138
Abstract: The genome of influenza A viruses comprises eight negative-strand RNA segments. Although all eight segments must be present in cells for efficient viral replication, the mechanism(s) by which these viral RNA (vRNA) segments are incorporated into virions is not fully understood. We recently found that sequences at both ends of the coding regions of the HA, NA, and NS vRNA segments of A/WSN/33 play important roles in the incorporation of these vRNAs into virions. In order to similarly identify the regions of the PB2, PB1, and PA vRNAs of this strain that are critical for their incorporation, we generated a series of mutant vRNAs that possessed the green fluorescent protein gene flanked by portions of the coding and noncoding regions of the respective segments. For all three polymerase segments, deletions at the ends of their coding regions decreased their virion incorporation efficiencies. More importantly, these regions not only affected the incorporation of the segment in which they reside, but were also important for the incorporation of other segments. This effect was most prominent with the PB2 vRNA. These findings suggest a hierarchy among vRNA segments for virion incorporation and may imply intersegment association of vRNAs during virus assembly.
Rights: Copyright © American Society for Microbiology.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 喜田 宏

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