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Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP

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Title: Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP
Authors: Noda, Takeshi Browse this author
Sagara, Hiroshi Browse this author
Suzuki, Emiko Browse this author
Takada, Ayato Browse this author →KAKEN DB
Kida, Hiroshi5 Browse this author →KAKEN DB
Kawaoka, Yoshihiro Browse this author →KAKEN DB
Authors(alt): 喜田, 宏5
Issue Date: May-2002
Publisher: American Society for Microbiology
Journal Title: Journal of Virology
Volume: 76
Issue: 10
Start Page: 4855
End Page: 4865
Publisher DOI: 10.1128/JVI.76.10.4855-4865.2002
PMID: 11967302
Abstract: Using biochemical assays, it has been demonstrated that expression of Ebola virus VP40 alone in mammalian cells induced production of particles with a density similar to that of virions. To determine the morphological properties of these particles, cells expressing VP40 and the particles released from the cells were examined by electron microscopy. VP40 induced budding from the plasma membrane of filamentous particles, which differed in length but had uniform diameters of approximately 65 nm. When the Ebola virus glycoprotein (GP) responsible for receptor binding and membrane fusion was expressed in cells, we found pleomorphic particles budding from the plasma membrane. By contrast, when GP was coexpressed with VP40, GP was found on the filamentous particles induced by VP40. These results demonstrated the central role of VP40 in formation of the filamentous structure of Ebola virions and may suggest an interaction between VP40 and GP in morphogenesis.
Rights: Copyright © American Society for Microbiology.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 喜田 宏

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